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AB174384

Anti-LexA DNA Binding Region antibody

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(6 Publications)

Rabbit Polyclonal LexA antibody. Suitable for WB, ChIP, IP, ICC/IF and reacts with Escherichia coli samples. Cited in 6 publications.

View Alternative Names

ExrA, LexA repressor, Tsl, UmuA

1 Images
Western blot - Anti-LexA DNA Binding Region antibody (AB174384)
  • WB

Supplier Data

Western blot - Anti-LexA DNA Binding Region antibody (AB174384)

All lanes:

Western blot - Anti-LexA DNA Binding Region antibody (ab174384) at 1/1000 dilution

All lanes:

E. coli whole cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB, IP, ChIP, ICC/IF

applications

Specificity

LexA protein of gram-negative bacteria and LexA fusion proteins for two-hybrid constructs

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Alternative Products

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AB234682

Anti-Streptococcal Pyrogenic Exotoxin B antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LexA DNA binding region is a component of the LexA protein also referred to simply as 'LexA'. LexA functions as a repressor protein in bacterial cells and has a molecular mass typically around 22.5 kDa. It is expressed in various bacterial species where it plays an important role in regulating DNA repair processes. LexA achieves its function by binding to specific DNA sequences and blocking transcription of genes involved in the SOS response.
Biological function summary

The LexA protein is critical in maintaining genomic integrity by controlling the expression of DNA repair genes. It is not usually part of a larger protein complex but operates independently within cells. In its inactive form LexA binds to the promoter regions of genes associated with the SOS response preventing their transcription. In response to DNA damage the RecA protein facilitates LexA's self-cleavage allowing for the induction of DNA repair genes.

Pathways

LexA is involved in the bacterial SOS response and DNA damage repair pathways. These pathways are vital for the organism's survival during genotoxic stress by coordinating the repair of damaged DNA. LexA works alongside other proteins such as RecA which senses DNA damage and aids in the self-cleavage of LexA releasing the suppression on SOS genes therefore initiating the repair process.

LexA's role is critical in bacterial resistance to antibiotics. The SOS response controlled by LexA can lead to increased mutation rates following antibiotic exposure potentially resulting in the development of antibiotic resistance. Understanding LexA's interactions with other proteins like RecA and its role in these processes could offer insights into more effective treatments to combat bacterial infections and reduce antibiotic resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LexA DNA Binding Region
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 10:e0067822 PubMed36169422

2022

Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Zlatkov,Moa Elsa Cecilia Näsman,Bernt Eric Uhlin

Virulence 13:698-713 PubMed35443872

2022

Extraintestinal pathogenic utilizes the surface-expressed elongation factor Tu to bind and acquire iron from holo-transferrin.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Xuhang Wang,Jin Li,Feng Xue,Fang Tang,Jianjun Dai

Frontiers in cellular and infection microbiology 10:592906 PubMed33569353

2021

Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic and Contributes to Opsonophagocytosis Resistance in Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Bin Xu,Xiangkai Zhuge,Fang Tang,Xuhang Wang,Qianwen Gong,Rui Chen,Feng Xue,Jianjun Dai

Cell reports 33:108527 PubMed33357439

2020

An RNA Repair Operon Regulated by Damaged tRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin J Hughes,Xinguo Chen,A Maxwell Burroughs,L Aravind,Sandra L Wolin

Nature communications 11:5643 PubMed33159083

2020

The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal.

Applications

Unspecified application

Species

Unspecified reactive species

Karol Kramarz,Kamila Schirmeisen,Virginie Boucherit,Anissia Ait Saada,Claire Lovo,Benoit Palancade,Catherine Freudenreich,Sarah A E Lambert

Genes & development 18:1886-97 PubMed15289460

2004

Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.

Applications

WB

Species

Escherichia coli

Takashi Hishida,Yong-Woon Han,Tatsuya Shibata,Yoshino Kubota,Yoshizumi Ishino,Hiroshi Iwasaki,Hideo Shinagawa
View all publications
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Product promise

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