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AB234695

Anti-LexA DNA Binding Region antibody

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(1 Publication)

Rabbit Polyclonal LexA antibody. Suitable for WB and reacts with Recombinant full length protein - Escherichia coli samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 lexA.

View Alternative Names

exrA, spr, tsl, umuA, b4043, JW4003, lexA, LexA repressor, ExrA, LexA repressor, Tsl, UmuA

1 Images
Western blot - Anti-LexA DNA Binding Region antibody (AB234695)
  • WB

Supplier Data

Western blot - Anti-LexA DNA Binding Region antibody (AB234695)

All lanes:

Western blot - Anti-LexA DNA Binding Region antibody (ab234695) at 1/500 dilution

Lane 1:

Recombinant Escherichia coli LexA Binding Region protein, 80 ng

Lane 2:

Recombinant Escherichia coli LexA Binding Region protein, 40 ng

Lane 3:

Recombinant Escherichia coli LexA Binding Region protein, 20 ng

Lane 4:

Recombinant Escherichia coli LexA Binding Region protein, 10 ng

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 49 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Escherichia coli K-12 lexA.

P0A7C2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Shigella flexneri": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LexA DNA binding region is a component of the LexA protein also referred to simply as 'LexA'. LexA functions as a repressor protein in bacterial cells and has a molecular mass typically around 22.5 kDa. It is expressed in various bacterial species where it plays an important role in regulating DNA repair processes. LexA achieves its function by binding to specific DNA sequences and blocking transcription of genes involved in the SOS response.
Biological function summary

The LexA protein is critical in maintaining genomic integrity by controlling the expression of DNA repair genes. It is not usually part of a larger protein complex but operates independently within cells. In its inactive form LexA binds to the promoter regions of genes associated with the SOS response preventing their transcription. In response to DNA damage the RecA protein facilitates LexA's self-cleavage allowing for the induction of DNA repair genes.

Pathways

LexA is involved in the bacterial SOS response and DNA damage repair pathways. These pathways are vital for the organism's survival during genotoxic stress by coordinating the repair of damaged DNA. LexA works alongside other proteins such as RecA which senses DNA damage and aids in the self-cleavage of LexA releasing the suppression on SOS genes therefore initiating the repair process.

LexA's role is critical in bacterial resistance to antibiotics. The SOS response controlled by LexA can lead to increased mutation rates following antibiotic exposure potentially resulting in the development of antibiotic resistance. Understanding LexA's interactions with other proteins like RecA and its role in these processes could offer insights into more effective treatments to combat bacterial infections and reduce antibiotic resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed : 20005847). The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed : 22412352). Genetic interactions among priB, dam, lexA, nagC, polA, rdgB, rdgB, rep and uup link the PriA-PriB replication restart pathway to DNA double-strand break repair (PubMed : 36326440).
See full target information lexA

Additional targets

LexA DNA Binding Region
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2020:5632342 PubMed33457194

2020

p300-Catalyzed Lysine Crotonylation Promotes the Proliferation, Invasion, and Migration of HeLa Cells via Heterogeneous Nuclear Ribonucleoprotein A1.

Applications

Unspecified application

Species

Unspecified reactive species

Xuesong Han,Xudong Xiang,Hongying Yang,Hongping Zhang,Shuang Liang,Jie Wei,Jing Yu
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Product promise

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