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AB246345

Anti-LGALS14 antibody [EPR9665] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LGALS14 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

PPL13, LGALS14, Placental protein 13-like, Charcot-Leyden crystal protein 2, Galectin-14, CLC2, Gal-14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)

Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150427)

Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)

Immunofluorescent analysis of JAR cells labelling LGALS14 with ab150427 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150427).

Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)

Intracellular Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150427)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9665

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab246345 is the carrier-free version of ab150427.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGALS14 also known as Galectin-14 is a member of the galectin family a group of beta-galactoside-binding proteins. It has a mass of around 14 kDa. This protein is expressed highly in eosinophils and placenta tissues. As a carbohydrate-binding protein LGALS14 participates in cellular interactions and its expression level may vary depending on cell activation states or pathological conditions.
Biological function summary

Galectin-14 functions in the immune response and regulation of inflammation. It can influence cell apoptosis and modulate signaling pathways through binding to specific carbohydrate moieties on cell surfaces. LGALS14 itself is not typically part of bigger protein complexes although it might interact with other galectins or proteins in certain cellular contexts to carry out its functions more effectively.

Pathways

This galectin family member holds a role in the immune system especially within pathways that manage inflammatory responses. It interacts with proteins such as Galectin-3 and Galectin-9 which also contribute to inflammatory regulation and immune function. These interactions help modulate immune signaling and processes like cell adhesion which are key components of the immune response pathway.

LGALS14 associates with conditions such as asthma and inflammatory bowel disease (IBD). In asthma Galectin-14 levels often correlate with eosinophil activity which is an indicator of inflammation severity. Furthermore research links LGALS14 with inflammation in IBD possibly through its role in modulating like Galectin-3 which also participate in inflammation pathways. Understanding its interactions and expression can provide insights into these diseases' mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.
See full target information LGALS14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 33:698-712 PubMed37662970

2023

Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Ruiz-Ciancio,Li-Hsien Lin,Suresh Veeramani,Maya N Barros,Diego Sanchez,Ary Lautaro Di Bartolo,Diego Masone,Paloma H Giangrande,María Belén Mestre,William H Thiel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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