Rabbit Recombinant Monoclonal LGALS14 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--immunocytochemistry-immunofluorescence-img98891.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)")
![Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--flow-cytometry-intracellular-img42466.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)")
![Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--immunocytochemistry-immunofluorescence-img66496.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (AB246345)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested | Tested |
| Mouse | Not recommended | Expected | Not recommended | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.
Alternative names
PPL13, LGALS14, Placental protein 13-like, Charcot-Leyden crystal protein 2, Galectin-14, CLC2, Gal-14
Recommended products
Rabbit Recombinant Monoclonal LGALS14 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9665
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246345 is the carrier-free version of Anti-LGALS14 antibody [EPR9665] ab150427.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LGALS14 also known as Galectin-14 is a member of the galectin family a group of beta-galactoside-binding proteins. It has a mass of around 14 kDa. This protein is expressed highly in eosinophils and placenta tissues. As a carbohydrate-binding protein LGALS14 participates in cellular interactions and its expression level may vary depending on cell activation states or pathological conditions.
- Biological function summary
Galectin-14 functions in the immune response and regulation of inflammation. It can influence cell apoptosis and modulate signaling pathways through binding to specific carbohydrate moieties on cell surfaces. LGALS14 itself is not typically part of bigger protein complexes although it might interact with other galectins or proteins in certain cellular contexts to carry out its functions more effectively.
- Pathways
This galectin family member holds a role in the immune system especially within pathways that manage inflammatory responses. It interacts with proteins such as Galectin-3 and Galectin-9 which also contribute to inflammatory regulation and immune function. These interactions help modulate immune signaling and processes like cell adhesion which are key components of the immune response pathway.
- Associated diseases and disorders
LGALS14 associates with conditions such as asthma and inflammatory bowel disease (IBD). In asthma Galectin-14 levels often correlate with eosinophil activity which is an indicator of inflammation severity. Furthermore research links LGALS14 with inflammation in IBD possibly through its role in modulating like Galectin-3 which also participate in inflammation pathways. Understanding its interactions and expression can provide insights into these diseases' mechanisms and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--immunocytochemistry-immunofluorescence-img98891.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)")
Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)
Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified Anti-LGALS14 antibody [EPR9665] ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LGALS14 antibody [EPR9665] ab150427)![Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--flow-cytometry-intracellular-img42466.jpg "Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)")
Flow Cytometry (Intracellular) - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)
Intracellular Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified Anti-LGALS14 antibody [EPR9665] ab150427 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LGALS14 antibody [EPR9665] ab150427)
![Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345), expandable thumbnail](https://content.abcam.com/products/images/lgals14-antibody-epr9665-bsa-and-azide-free-ab246345--immunocytochemistry-immunofluorescence-img66496.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)")
Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] - BSA and Azide free (ab246345)
Immunofluorescent analysis of JAR cells labelling LGALS14 with Anti-LGALS14 antibody [EPR9665] ab150427 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LGALS14 antibody [EPR9665] ab150427).
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