Rabbit Recombinant Monoclonal LGALS3BP antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img59045.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572)")
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img89626.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572)")
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img16445.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (AB217572)")
![Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (AB217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--flow-cytometry-intracellular-img413982.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (AB217572)")
![Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (AB217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--flow-cytometry-intracellular-img415069.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (AB217572)")
Publications
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- Science advances 9:eadd81642023Non-cell-autonomous regulation of interneuron specification mediated by extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabrizia Pipicelli,Natalia Baumann,Rossella Di Giaimo,Andrea Forero-Echeverry,Christina Kyrousi,Rebecca Bonrath,Giuseppina Maccarrone,Denis Jabaudon,Silvia CappelloPubMed 37205765
- Nature communications 12:34062021SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClemens Gutmann,Kaloyan Takov,Sean A Burnap,Bhawana Singh,Hashim Ali,Konstantinos Theofilatos,Ella Reed,Maria Hasman,Adam Nabeebaccus,Matthew Fish,Mark Jw McPhail,Kevin O'Gallagher,Lukas E Schmidt,Christian Cassel,Marieke Rienks,Xiaoke Yin,Georg Auzinger,Salvatore Napoli,Salma F Mujib,Francesca Trovato,Barnaby Sanderson,Blair Merrick,Umar Niazi,Mansoor Saqi,Konstantina Dimitrakopoulou,Rafael Fernández-Leiro,Silke Braun,Romy Kronstein-Wiedemann,Katie J Doores,Jonathan D Edgeworth,Ajay M Shah,Stefan R Bornstein,Torsten Tonn,Adrian C Hayday,Mauro Giacca,Manu Shankar-Hari,Manuel MayrPubMed 34099652
- Heliyon 6:e058042020Identification of novel substrates of a disintegrin and metalloprotease 17 by specific labeling of surface proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuki Omoteyama et. al.PubMed 33385093
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Alternative names
M2BP, LGALS3BP, Galectin-3-binding protein, Basement membrane autoantigen p105, Lectin galactoside-binding soluble 3-binding protein, Mac-2-binding protein, Tumor-associated antigen 90K, MAC2BP, Mac-2 BP
Recommended products
Rabbit Recombinant Monoclonal LGALS3BP antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR21757-33
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LGALS3BP also known as Galectin-3-binding protein (Mac-2 binding protein 90K) is a multifaceted glycoprotein with a mass of approximately 90 kDa. It typically interacts with galectins especially galectin-3 and is known for its adhesive properties. Researchers frequently observe it in various tissues including epithelial cells immune cells and in the extracellular matrix highlighting its involvement across different biological functions.
- Biological function summary
The LGALS3BP glycoprotein modulates cell-cell and cell-matrix interactions. It does not operate alone; researchers have identified its presence in multiple complexes impacting several cellular processes such as immune modulation cell adhesion and migration. LGALS3BP can affect the immune response by influencing T-cell and natural killer cell activities which can alter the body's defense against pathogens and tumors.
- Pathways
LGALS3BP significantly influences the signaling pathways involved with immune function and cellular adhesion. It plays an important role in the galectin pathway where it interacts with galectin-3 contributing to processes like cell growth and apoptosis. Additionally it affects the integrin-mediated signaling pathway enhancing cell adhesion and motility which is vital for cell migration and signaling dynamics. These interactions involve proteins such as integrins and other adhesion molecules that influence cellular communication and structural integrity.
- Associated diseases and disorders
LGALS3BP is strongly linked to cancer and inflammation-related conditions. Its overexpression is correlated with tumor progression and metastasis as seen in various cancers such as breast and colorectal cancer. LGALS3BP modulates its effects by interacting with galectin-3 influencing tumor-immune escape mechanisms and promoting tumor growth. Further its role in inflammatory disorders suggests it contributes to pathological processes through interactions with proteins that mediate inflammation and immune response.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img59045.jpg "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)")
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
LGALS3BP Western blot staining using rabbit Anti-LGALS3BP antibody
Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 10 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
All lanes: Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa, 97 kDa
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img89626.jpg "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)")
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
LGALS3BP Western blot staining using rabbit Anti-LGALS3BP antibody
Blocking/Dilution buffer: 5% NFDM/TBST.Exposure times: Lane 1: 15 seconds; Lanes 3 and 4: 136 seconds; Lane 4: 3 minutes; Lane 5: 15 seconds; Lanes 6,7,8 and 9: 103 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
All lanes: Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1: Rat brain lysate at 10 µg
Lane 2: Rat liver lysate at 10 µg
Lane 3: Rat spleen lysate at 10 µg
Lane 4: Mouse heart lysate at 10 µg
Lane 5: Mouse kidney lysate at 10 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate at 10 µg
Lane 7: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg
Lane 8: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 9: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa, 97 kDa
![Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--western-blot-img16445.jpg "Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)")
Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
LGALS3BP Western blot staining using rabbit Anti-LGALS3BP antibody
Blocking/Dilution buffer: 5% NFDM/TBST.Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 15 seconds; Lanes 5 and 6: 81 seconds.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21526146, PMID: 21094132).
Lanes 1 - 4: Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/5000 dilution
Lanes 5 - 6: Western blot - Anti-LGALS3BP antibody [EPR21757-33] (ab217572) at 1/1000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa, 97 kDa
![Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--flow-cytometry-intracellular-img413982.jpg "Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)")
Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572), expandable thumbnail](https://content.abcam.com/products/images/lgals3bp-antibody-epr21757-33-ab217572--flow-cytometry-intracellular-img415069.jpg "Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)")
Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] (ab217572)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
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