JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317149

Anti-LGALS3BP antibody [HL2168]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal LGALS3BP antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3BP.

View Alternative Names

M2BP, LGALS3BP, Galectin-3-binding protein, Basement membrane autoantigen p105, Lectin galactoside-binding soluble 3-binding protein, Mac-2-binding protein, Tumor-associated antigen 90K, MAC2BP, Mac-2 BP

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [HL2168] (AB317149)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [HL2168] (AB317149)

ab317149 detects LGALS3BP protein at endoplasmic reticulum and cell junction by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : LGALS3BP stained by ab317149 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [HL2168] (AB317149)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [HL2168] (AB317149)

ab317149 detects LGALS3BP protein at cytoplasmic vesicle by immunofluorescent analysis. Sample : A431 cells were fixed in ice-cold MeOH for 5 min. Green : LGALS3BP stained by ab317149 diluted at 1 : 500. Red : alpha Tubulin a cytoskeleton marker stained by alpha Tubulin antibody [GT114] diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [HL2168] (AB317149)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [HL2168] (AB317149)

ab317149 detects secreted LGALS3BP protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse adrenal gland. LGALS3BP stained by ab317149 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-LGALS3BP antibody [HL2168] (AB317149)
  • WB

Supplier Data

Western blot - Anti-LGALS3BP antibody [HL2168] (AB317149)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317149 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-LGALS3BP antibody [HL2168] (ab317149) at 1/1000 dilution

Lane 2:

HL-60 whole cell extracts at 30 µg

Lane 3:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-LGALS3BP antibody [HL2168] (AB317149)
  • WB

Supplier Data

Western blot - Anti-LGALS3BP antibody [HL2168] (AB317149)

Untreated (–) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317149 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-LGALS3BP antibody [HL2168] (ab317149) at 1/1000 dilution

Lane 1:

Untreated (–) A431 whole cell extracts at 30 µg

Lane 2:

24 ug/mL Tunicamycin, 16 hr treated (+) A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2168

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LGALS3BP.

Q08380

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGALS3BP also known as Galectin-3-binding protein (Mac-2 binding protein 90K) is a multifaceted glycoprotein with a mass of approximately 90 kDa. It typically interacts with galectins especially galectin-3 and is known for its adhesive properties. Researchers frequently observe it in various tissues including epithelial cells immune cells and in the extracellular matrix highlighting its involvement across different biological functions.
Biological function summary

The LGALS3BP glycoprotein modulates cell-cell and cell-matrix interactions. It does not operate alone; researchers have identified its presence in multiple complexes impacting several cellular processes such as immune modulation cell adhesion and migration. LGALS3BP can affect the immune response by influencing T-cell and natural killer cell activities which can alter the body's defense against pathogens and tumors.

Pathways

LGALS3BP significantly influences the signaling pathways involved with immune function and cellular adhesion. It plays an important role in the galectin pathway where it interacts with galectin-3 contributing to processes like cell growth and apoptosis. Additionally it affects the integrin-mediated signaling pathway enhancing cell adhesion and motility which is vital for cell migration and signaling dynamics. These interactions involve proteins such as integrins and other adhesion molecules that influence cellular communication and structural integrity.

LGALS3BP is strongly linked to cancer and inflammation-related conditions. Its overexpression is correlated with tumor progression and metastasis as seen in various cancers such as breast and colorectal cancer. LGALS3BP modulates its effects by interacting with galectin-3 influencing tumor-immune escape mechanisms and promoting tumor growth. Further its role in inflammatory disorders suggests it contributes to pathological processes through interactions with proteins that mediate inflammation and immune response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
See full target information LGALS3BP
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com