AB236509

Anti-LGALS3BP antibody [OTI3D6]

Name: Anti-LGALS3BP antibody [OTI3D6] (ab236509) | Abcam
Brand: Abcam Limited
SKU: AB236509
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal LGALS3BP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3BP aa 1-300.

View Alternative Names

M2BP, LGALS3BP, Galectin-3-binding protein, Basement membrane autoantigen p105, Lectin galactoside-binding soluble 3-binding protein, Mac-2-binding protein, Tumor-associated antigen 90K, MAC2BP, Mac-2 BP

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Paraffin-embedded human lung carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Paraffin-embedded human bladder carcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with empty vector control (blue) or vector expressing LGALS3BP (red), labeling LGALS3BP with ab236509 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Paraffin-embedded human prostate tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for LGALS3BP using ab236509 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LGALS3BP cDNA stained for LGALS3BP (green) using ab236509 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

All lanes:

Western blot - Anti-LGALS3BP antibody [OTI3D6] (ab236509) at 1/500 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate at 5 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY LGALS3BP cDNA, whole cell lysate at 5 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3D6

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human LGALS3BP aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q08380

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat induced epitope retrieval by 10mM citric buffer, pH 6.0, 100°C for 10 minutes.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Clone OTI3D6 (formerly 3D6).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGALS3BP also known as Galectin-3-binding protein (Mac-2 binding protein 90K) is a multifaceted glycoprotein with a mass of approximately 90 kDa. It typically interacts with galectins especially galectin-3 and is known for its adhesive properties. Researchers frequently observe it in various tissues including epithelial cells immune cells and in the extracellular matrix highlighting its involvement across different biological functions.

Biological function summary

The LGALS3BP glycoprotein modulates cell-cell and cell-matrix interactions. It does not operate alone; researchers have identified its presence in multiple complexes impacting several cellular processes such as immune modulation cell adhesion and migration. LGALS3BP can affect the immune response by influencing T-cell and natural killer cell activities which can alter the body's defense against pathogens and tumors.

Pathways

LGALS3BP significantly influences the signaling pathways involved with immune function and cellular adhesion. It plays an important role in the galectin pathway where it interacts with galectin-3 contributing to processes like cell growth and apoptosis. Additionally it affects the integrin-mediated signaling pathway enhancing cell adhesion and motility which is vital for cell migration and signaling dynamics. These interactions involve proteins such as integrins and other adhesion molecules that influence cellular communication and structural integrity.

LGALS3BP is strongly linked to cancer and inflammation-related conditions. Its overexpression is correlated with tumor progression and metastasis as seen in various cancers such as breast and colorectal cancer. LGALS3BP modulates its effects by interacting with galectin-3 influencing tumor-immune escape mechanisms and promoting tumor growth. Further its role in inflammatory disorders suggests it contributes to pathological processes through interactions with proteins that mediate inflammation and immune response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

See full target information LGALS3BP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 26:222 PubMed39702240

2024

KLF2 controls the apoptosis of neutrophils and is associated with disease activity of systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshuai Zhao,Zaichuan Lin,Peiwen Zhang,Jiayue Rao,Shumin Xu,Qing Luo,Junming Li

eLife 12: PubMed37010266

2023

Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Xinqi Lou,Yingjie Zha,Yinyin Qin,Jun Zha,Lei Hong,Zhanli Xie,Shudi Yang,Chen Wang,Jianzhong An,Zhenhao Zhang,Shigang Qiao

Investigative ophthalmology & visual science 63:25 PubMed35895036

2022

LGALS3BP in Microglia Promotes Retinal Angiogenesis Through PI3K/AKT Pathway During Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Zhao,Yusen Liu,Jiayu Meng,Xiaotang Wang,Xianyang Liu,Wanqian Li,Qian Zhou,Junjie Xiang,Na Li,Shengping Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGALS3BP antibody [OTI3D6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Immunocytochemistry/ Immunofluorescence - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Western blot - Anti-LGALS3BP antibody [OTI3D6] (AB236509)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise