AB318293

Anti-LGB antibody [EPR26585-15] - BSA and Azide free (Detector)

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal LGB antibody. Carrier free. Suitable for sELISA and reacts with Cow samples.

View Alternative Names

Beta-lactoglobulin, Beta-LG, LGB

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Sandwich ELISA - Anti-LGB antibody [EPR26585-15] - BSA and Azide free (Detector) (AB318293)

sELISA

Supplier Data

Sandwich ELISA - Anti-LGB antibody [EPR26585-15] - BSA and Azide free (Detector) (AB318293)

Sandwich ELISA of ab318291 with the capture antibody ab318292 dilution at 2 ug/mL and detector antibody ab318293 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26585-15

Isotype

IgG

Carrier free

Yes

Reacts with

Cow

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cow": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab318293 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Bovine LGB. The recommended pair for sandwich ELISA is:

Capture: ab318292, Bovine LGB Capture Antibody (unconjugated)

Detector: ab318293, Bovine LGB Detector Antibody (unconjugated)

The reference range value is 7.81-1000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGB also known as beta-lactoglobulin is a small globular protein with a mass of approximately 18 kDa. It is predominantly expressed in the milk of ruminants including cows sheep and goats. The protein is considered a major whey protein and it forms small soluble aggregates in milk. LGB exhibits the ability to bind hydrophobic molecules which suggests it plays a role in transport or storage of lipophilic molecules within milk.

Biological function summary

This protein functions in the stabilization and transport of fatty acids and other hydrophobic molecules. LGB does not form part of a larger protein complex but can dimerize under certain conditions. It has a notable affinity for binding ligands particularly vitamins and flavor compounds which may significantly influence nutritional and sensory qualities of milk and dairy products.

Pathways

LGB's functional role suggests involvement in metabolic and nutritional pathways related to lipid transport and digestion. It has an indirect relationship with the digestive enzyme lipase as LGB-bound lipids become more accessible for digestion. Furthermore its lipid-binding characteristics support its involvement with fatty acid metabolism playing a role similar to serum albumin in circulating lipid constituents.

LGB is most commonly implicated in food allergies particularly cow's milk allergy. Its antigenic properties make it a significant allergen in humans especially young children. Through this context LGB relates to the immune response proteins such as immunoglobulin E (IgE) which mediate allergic reactions. Understanding these interactions between LGB and immune components remains essential for developing hypoallergenic milk products.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

See full target information LGB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGB antibody [EPR26585-15] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-LGB antibody [EPR26585-15] - BSA and Azide free (Detector) (AB318293)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise