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AB228613

Anti-Lgi1/EPT antibody

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(1 Publication)

Rabbit Polyclonal Lgi1/EPT antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LGI1.

View Alternative Names

EPT, UNQ775/PRO1569, LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Lgi1/EPT antibody (AB228613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lgi1/EPT antibody (AB228613)

4% paraformaldehyde-fixed DIV10 rat E18 primary hippocampal neurons stained for Lgi1/EPT (green) using ab228613 at 1/500 dilution in ICC/IF.

Nuclear counter stain : DAPI (blue). beta Tubulin 3 was stained with an anti-beta Tubulin 3 antibody at 1/500 dilution.

Western blot - Anti-Lgi1/EPT antibody (AB228613)
  • WB

Supplier Data

Western blot - Anti-Lgi1/EPT antibody (AB228613)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Lgi1/EPT antibody (ab228613) at 1/5000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LGI1. The exact immunogen used to generate this antibody is proprietary information.

O95970

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lgi1 also known as leucine-rich glioma inactivated 1 is a synaptic protein with a mass of approximately 60 kDa. This protein is notably expressed in the brain where it plays a mechanical role in interacting with other synaptic molecules to mediate the formation and function of synaptic junctions. Lgi1 mainly associates with neuronal cell surfaces and is critical in synaptic stability and neurotransmitter release.
Biological function summary

Lgi1 influences synaptic transmission and plasticity. It forms a complex with the ADAM22 and ADAM23 proteins which are part of the disintegrin and metalloproteinase domain family. This complex regulates the activity and localization of ion channels involved in neuronal excitability influencing electrical signaling across neurons. These interactions are essential for maintaining proper neuronal communication and cognitive function.

Pathways

Several biological processes involve Lgi1 including the regulation of the voltage-gated potassium channel pathways and synaptic signaling pathways. Specifically Lgi1's interaction with ADAM22 facilitates the assembly of the postsynaptic density therefore contributing to synaptic strength. Moreover Lgi1 interacts with Kv1.1 an important partner in the modulation of ion channel activities that are central to neuronal signaling pathways.

Several conditions have a connection to Lgi1 especially autoimmune encephalitis and epilepsy. Autoimmune encephalitis often involves antibodies targeting Lgi1 affecting synaptic transmission and resulting in neurological symptoms. In epilepsy Lgi1 abnormalities potentially through disrupted interactions with related proteins like ADAM22 can lead to imbalances in neuronal excitability and trigger seizures. Understanding these connections helps in developing targeted therapies for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
See full target information LGI1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurology(R) neuroimmunology & neuroinflammation 10: PubMed37591767

2023

Electrolyte Imbalance in Anti-LGI1 Encephalitis: It Is Not All in Your Head.

Applications

Unspecified application

Species

Unspecified reactive species

Avi Gadoth,Michal Nisnboym,Yifat Alcalay,Asia Zubkov,Idit Schwartz,Doron Schwartz,Marana Abboud,Tamar Rubinek,Ofer Yossepowitch,Talia Weinstein
View all publications
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Product promise

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