Skip to main content

Rabbit Recombinant Monoclonal Lgi1/EPT antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Lgi1/EPT antibody [EPR9084] (AB137045), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (AB137045), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (AB137045), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (AB137045), expandable thumbnail
  • Western blot - Anti-Lgi1/EPT antibody [EPR9084] (AB137045), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/300
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100 - 1/300
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/300
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.

Alternative names

EPT, UNQ775/PRO1569, LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin-1

Recommended products

Rabbit Recombinant Monoclonal Lgi1/EPT antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9084
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lgi1 also known as leucine-rich glioma inactivated 1 is a synaptic protein with a mass of approximately 60 kDa. This protein is notably expressed in the brain where it plays a mechanical role in interacting with other synaptic molecules to mediate the formation and function of synaptic junctions. Lgi1 mainly associates with neuronal cell surfaces and is critical in synaptic stability and neurotransmitter release.

Biological function summary

Lgi1 influences synaptic transmission and plasticity. It forms a complex with the ADAM22 and ADAM23 proteins which are part of the disintegrin and metalloproteinase domain family. This complex regulates the activity and localization of ion channels involved in neuronal excitability influencing electrical signaling across neurons. These interactions are essential for maintaining proper neuronal communication and cognitive function.

Pathways

Several biological processes involve Lgi1 including the regulation of the voltage-gated potassium channel pathways and synaptic signaling pathways. Specifically Lgi1's interaction with ADAM22 facilitates the assembly of the postsynaptic density therefore contributing to synaptic strength. Moreover Lgi1 interacts with Kv1.1 an important partner in the modulation of ion channel activities that are central to neuronal signaling pathways.

Associated diseases and disorders

Several conditions have a connection to Lgi1 especially autoimmune encephalitis and epilepsy. Autoimmune encephalitis often involves antibodies targeting Lgi1 affecting synaptic transmission and resulting in neurological symptoms. In epilepsy Lgi1 abnormalities potentially through disrupted interactions with related proteins like ADAM22 can lead to imbalances in neuronal excitability and trigger seizures. Understanding these connections helps in developing targeted therapies for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    All lanes: Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045) at 1/1000 dilution

    Lane 1: Human brain lysate at 10 µg

    Lane 2: HeLa lysate at 10 µg

    Lane 3: U87-MG lysate at 10 µg

    Secondary

    All lanes: HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 64 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Lgi1/EPT with ab137045 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Low expression: heart, kidney, spleen.
    The identity of the bands lower than 25 kDa is unknown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Immunohistochemical analysis of paraffin-embedded rat kidney labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on rat kidney.

    The section was incubated with ab137045 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Low expression: heart, kidney, spleen.

    The identity of the bands lower than 25 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Lgi1/EPT antibody [EPR9084] (ab137045) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 64 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse cerebrum.

    The section was incubated with ab137045 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Immunohistochemical analysis of paraffin-embedded mouse kidney labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse kidney.

    The section was incubated with ab137045 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] (ab137045)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in rat cerebrum.

    The section was incubated with ab137045 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com