AB115305

Anti-Lgi4 antibody

Name: Anti-Lgi4 antibody (ab115305) | Abcam
Brand: Abcam Limited
SKU: AB115305

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(1 Publication)

Rabbit Polyclonal LGI4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LGI4.

View Alternative Names

LGIL3, UNQ6515/PRO21485, LGI4, Leucine-rich repeat LGI family member 4, LGI1-like protein 3, Leucine-rich glioma-inactivated protein 4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

ab115305, at 5µg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Heart tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

ab115305, at 5µg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Skeletal Muscle tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

ab115305, at 5μg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-Lgi4 antibody (AB115305)

Lane 1:

Western blot - Anti-Lgi4 antibody (ab115305) at 1 µg/mL

Lane 2:

Western blot - Anti-Lgi4 antibody (ab115305) at 2 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LGI4. The exact immunogen used to generate this antibody is proprietary information.

Q8N135

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lgi4 also known as leucine-rich glioma inactivated 4 or Lgi-4 is a member of the Lgi protein family. This protein has an approximate mass of 120 kDa. Lgi4 plays an important structural role contributing to cell-cell communication in the nervous system. It is expressed in the peripheral nervous tissue specifically in Schwann cells which are part of the myelination process of peripheral nerves.

Biological function summary

Lgi4 participates in Schwann cell proliferation and differentiation impacting the myelination of peripheral nerves. As part of the non-covalent complex involving other Lgi family members it ensures the structural integrity and function of the myelin sheath. It regulates the interactions between neurons and glial cells promoting proper nerve function and development.

Pathways

Lgi4 interacts with the Nogo and Notch signaling pathways which are important for nerve growth and regeneration. In these pathways Lgi4 establishes interactions with Nogo receptor proteins and Notch receptors modulating signal transmission and myelin formation. These pathways play significant roles in neural development and repairing processes.

Lgi4 has associations with Charcot-Marie-Tooth disease and peripheral neuropathy. Mutations or dysregulation of Lgi4 can lead to defective myelination contributing to the pathology of these conditions. Its connection with neuregulins and myelin protein zero is important as these proteins are also involved in the myelination defects observed in these disorders. This highlights Lgi4's role in maintaining neural health and potential therapeutic interest.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation (By similarity).

See full target information LGI4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 17:5362-5367 PubMed29393450

2018

Cullin7 promotes epithelial‑mesenchymal transition of esophageal carcinoma via the ERK‑SNAI2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Tian,Dandan Liu,Luyi Sun,Hui Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lgi4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi4 antibody (AB115305)

Western blot - Anti-Lgi4 antibody (AB115305)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise