AB109594

Anti-LGN antibody

Name: Anti-LGN antibody (ab109594) | Abcam
Brand: Abcam Limited
SKU: AB109594
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal LGN antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GPSM2 aa 600 to C-terminus.

View Alternative Names

LGN, GPSM2, G-protein-signaling modulator 2, Mosaic protein LGN

2 Images

Unknown

Immunoprecipitation - Anti-LGN antibody (AB109594)

Detection of LGN by Western Blot of Immunprecipitate.
ab109594 at 1μg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LGN antibody (ab109594)

Predicted band size: 77 kDa

false

Unknown

Western blot - Anti-LGN antibody (AB109594)

All lanes:

Western blot - Anti-LGN antibody (ab109594) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GPSM2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P81274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGN also known as GPSM2 (G-protein signaling modulator 2) is a protein involved in cellular processes related to signaling and cell polarity. It has a molecular mass of approximately 80 kDa. LGN is expressed in various tissues including the central nervous system where it plays a role in neural cell functions. This protein acts mechanically by interacting with G-proteins and aiding in proper signal transduction.

Biological function summary

The protein LGN influences cell division by regulating spindle orientation which is essential for accurate cell fate determination. LGN is a part of a complex with the proteins NuMA (nuclear mitotic apparatus protein) and dynein-dynactin which assists in the positioning of the mitotic spindle during cellular division. This activity ensures that cells divide correctly maintaining tissue organization and function.

Pathways

LGN is important in asymmetric cell division and cellular signaling. It is involved in the Wnt signaling pathway which is known for its role in cell growth and development. Additionally LGN interacts with G-proteins modulating their activity to facilitate proper signal transduction within these pathways. The influences on these pathways highlight the protein's necessity in maintaining cellular organization and signaling fidelity.

LGN is linked to certain neurodevelopmental disorders such as microcephaly where patients exhibit reduced brain size due to abnormal cell division. It also connects to certain types of cancer through its role in maintaining correct spindle orientation and polarity which is important for preventing uncontrolled cell proliferation. LGN's interaction with proteins like NuMA and G-proteins remains a focal point in understanding its impact on these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in mitotic spindle pole organization via its interaction with NUMA1 (PubMed : 11781568, PubMed : 15632202, PubMed : 21816348). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364). Plays a role in metaphase spindle orientation (PubMed : 22327364). Also plays an important role in asymmetric cell divisions (PubMed : 21816348). Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (By similarity).

See full target information GPSM2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100002 PubMed35113786

2022

Long non-coding RNA DLGAP1 antisense RNA 1 accelerates glioma progression via the microRNA-628-5p/DEAD-box helicase 59 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Qi Hu,Xiang-Sheng Ao

EMBO reports 20:e47097 PubMed31321879

2019

Proteostasis is essential during cochlear development for neuron survival and hair cell polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Freeman,Susana Mateo Sánchez,Ronald Pouyo,Pierre-Bernard Van Lerberghe,Kevin Hanon,Nicolas Thelen,Marc Thiry,Giovanni Morelli,Laura Van Hees,Sophie Laguesse,Alain Chariot,Laurent Nguyen,Laurence Delacroix,Brigitte Malgrange

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGN antibody

Immunoprecipitation - Anti-LGN antibody (AB109594)

Western blot - Anti-LGN antibody (AB109594)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise