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AB110147

Anti-LGN antibody

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(1 Publication)

Rabbit Polyclonal LGN antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GPSM2 aa 450-500.

View Alternative Names

LGN, GPSM2, G-protein-signaling modulator 2, Mosaic protein LGN

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Immunoprecipitation - Anti-LGN antibody (AB110147)
  • IP

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Immunoprecipitation - Anti-LGN antibody (AB110147)

Detection of LGN in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab110147 at 6 μg/mg lysate for IP (Lane 1). For WB detection an anti-LGN antibody which recognizes a downstream epitope was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LGN antibody (ab110147)

Predicted band size: 77 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human GPSM2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

P81274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGN also known as GPSM2 (G-protein signaling modulator 2) is a protein involved in cellular processes related to signaling and cell polarity. It has a molecular mass of approximately 80 kDa. LGN is expressed in various tissues including the central nervous system where it plays a role in neural cell functions. This protein acts mechanically by interacting with G-proteins and aiding in proper signal transduction.
Biological function summary

The protein LGN influences cell division by regulating spindle orientation which is essential for accurate cell fate determination. LGN is a part of a complex with the proteins NuMA (nuclear mitotic apparatus protein) and dynein-dynactin which assists in the positioning of the mitotic spindle during cellular division. This activity ensures that cells divide correctly maintaining tissue organization and function.

Pathways

LGN is important in asymmetric cell division and cellular signaling. It is involved in the Wnt signaling pathway which is known for its role in cell growth and development. Additionally LGN interacts with G-proteins modulating their activity to facilitate proper signal transduction within these pathways. The influences on these pathways highlight the protein's necessity in maintaining cellular organization and signaling fidelity.

LGN is linked to certain neurodevelopmental disorders such as microcephaly where patients exhibit reduced brain size due to abnormal cell division. It also connects to certain types of cancer through its role in maintaining correct spindle orientation and polarity which is important for preventing uncontrolled cell proliferation. LGN's interaction with proteins like NuMA and G-proteins remains a focal point in understanding its impact on these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in mitotic spindle pole organization via its interaction with NUMA1 (PubMed : 11781568, PubMed : 15632202, PubMed : 21816348). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed : 22327364). Plays a role in metaphase spindle orientation (PubMed : 22327364). Also plays an important role in asymmetric cell divisions (PubMed : 21816348). Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity (By similarity).
See full target information GPSM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of comparative neurology 528:2161-2173 PubMed32037591

2020

Smad anchor for receptor activation nuclear localization during development identifies Layers V and VI of the neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Mestres,Maximilian Einsiedel,June Möller,Beatriz Cardoso de Toledo
View all publications
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