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AB314009

Anti-LGR5 antibody [4H8]

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Rabbit Monoclonal LGR5 antibody. Suitable for WB, Flow Cyt and reacts with Human, Rat samples. Immunogen corresponding to Synthetic Peptide within Human LGR5.

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

2 Images
Flow Cytometry - Anti-LGR5 antibody [4H8] (AB314009)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LGR5 antibody [4H8] (AB314009)

Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab314009 (pink line) at 1 : 50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1x10E6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1μg/1x10E6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western blot - Anti-LGR5 antibody [4H8] (AB314009)
  • WB

Supplier Data

Western blot - Anti-LGR5 antibody [4H8] (AB314009)

All lanes:

Western blot - Anti-LGR5 antibody [4H8] (ab314009) at 1/500 dilution

Lane 1:

SH-SY5Y whole cell lysate

Lane 2:

Rat brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

4H8

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human LGR5.

O75473

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.
Biological function summary

LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.

Pathways

LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.

LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
See full target information LGR5

Additional targets

Lgr5
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Product promise

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