AB219107

Anti-LGR5 antibody

Name: Anti-LGR5 antibody (ab219107) | Abcam
Brand: Abcam Limited
SKU: AB219107
Price: 485 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(14 Publications)

Anti-LGR5 antibody (ab219107) is a rabbit polyclonal antibody detecting LGR5 in IHC-P. Suitable for Human.

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB219107)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB219107)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas, islets tissue labeling LGR5 with ab219107 at 1/400 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human LGR5. The exact immunogen used to generate this antibody is proprietary information.

O75473

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-LGR5 antibody (ab219107) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.

Biological function summary

LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.

Pathways

LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as Β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.

LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

See full target information LGR5

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 46:13798-13810 PubMed39727953

2024

Analysis of Cancer Stem Cell Markers in Various Histological Subtypes of Adrenocortical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nano Pachuashvili,Asya Bastrich,Erika Porubayeva,Alina Elfimova,Alexander Tertychnyy,Dmitry Beltsevich,Evgeniya Kogan,Igor Reshetov,Ekaterina Troshina,Natalia Tarbaeva,Natalia Mokrysheva,Liliya Urusova

International journal of molecular sciences 25: PubMed39684417

2024

Anorectal Remodeling in the Transitional Zone with Increased Expression of LGR5, SOX9, SOX2, and Keratin 13 and 5 in a Dextran Sodium Sulfate-Induced Mouse Model of Ulcerative Colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Kobayashi,Tatsuya Usui,Mohamed Elbadawy,Tetsuhito Kigata,Masahiro Kaneda,Tomoaki Murakami,Takuma Kozono,Yoshiyuki Itoh,Makoto Shibutani,Toshinori Yoshida

Poultry science 103:104487 PubMed39531804

2024

The vital role of melatonin in the intestinal mucosal barrier integrity in chick.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gao,Chao Song,Zixu Wang,Yaoxing Chen

Pharmaceutics 16: PubMed39065640

2024

Antitumor Activity of a Pyrrolobenzodiazepine Antibody-Drug Conjugate Targeting LGR5 in Preclinical Models of Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianghua Tu,Yukimatsu Toh,Adela M Aldana,Jake J Wen,Ling Wu,Joan Jacob,Li Li,Sheng Pan,Kendra S Carmon,Qingyun J Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403075 PubMed39041890

2024

Biomimetic Supramolecular Assembly with IGF-1C Delivery Ameliorates Inflammatory Bowel Disease (IBD) by Restoring Intestinal Barrier Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Enze Fu,Meng Qian,Ningning He,Yilun Yin,Yue Liu,Zhibo Han,ZhongChao Han,Qiang Zhao,Xiaocang Cao,Zongjin Li

Heliyon 10:e30330 PubMed38726177

2024

NAT10-mediated acC modification promotes stemness and chemoresistance of colon cancer by stabilizing NANOGP8.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Gao,Ting-Dong Li,Su-Zhen Yang,Hui-Min Ma,Xiang Wang,De-Kui Zhang

Cell death discovery 10:164 PubMed38575595

2024

Hypoxic preconditioning accelerates the healing of ischemic intestinal injury by activating HIF-1α/PPARα pathway-mediated fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Linxia Li,Yanqi Liu,Na Zhi,Yaoxuan Ji,Jialing Xu,Guoyun Mao,Yazhou Wang,Jin Ma,Yunying Wang

Nature communications 15:2526 PubMed38514666

2024

Trimethylamine N-oxide impairs β-cell function and glucose tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Kong,Qijin Zhao,Xiaojing Jiang,Jinping Hu,Qian Jiang,Li Sheng,Xiaohong Peng,Shusen Wang,Yibing Chen,Yanjun Wan,Shaocong Hou,Xingfeng Liu,Chunxiao Ma,Yan Li,Li Quan,Liangyi Chen,Bing Cui,Pingping Li

Science advances 9:eadi6488 PubMed37967178

2023

Promotion of uterine reconstruction by a tissue-engineered uterus with biomimetic structure and extracellular matrix microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Long-Mei Zhao,Lin-Cui Da,Rui Wang,Long Wang,Yan-Lin Jiang,Xiu-Zhen Zhang,Ya-Xing Li,Xiong-Xin Lei,Yu-Ting Song,Chen-Yu Zou,Li-Ping Huang,Wen-Qian Zhang,Qing-Yi Zhang,Qian-Jin Li,Rong Nie,Yi Zhang,Yan Liang,Jesse Li-Ling,Hui-Qi Xie

BMC cancer 23:1088 PubMed37950151

2023

Circular RNA ZNF800 (hsa_circ_0082096) regulates cancer stem cell properties and tumor growth in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Vimalan Rengganaten,Chiu-Jung Huang,Mong-Lien Wang,Yueh Chien,Ping-Hsing Tsai,Yuan-Tzu Lan,Hooi Tin Ong,Shih-Hwa Chiou,Kong Bung Choo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGR5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB219107)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise