AB327149

Anti-LGR5 Antibody [CL13567]

Name: Anti-LGR5 Antibody [CL13567] (ab327149) | Abcam
Brand: Abcam Limited
SKU: AB327149
Price: 580 USD
Availability: InStock

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Mouse Monoclonal LGR5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LGR5.

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human ovarian cancer (high grade serous ovarian carcinoma) using ab327149 at 1 : 5000–1 : 10000 dilution, showing strong cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemistry analysis in human fallopian tube and tonsil tissues using ab327149 at 1 : 5000–1 : 10000 dilution. Corresponding LGR5 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human skeletal muscle using ab327149 at 1 : 5000–1 : 10000 dilution, showing moderate membranous positivity in myocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human fallopian tube using ab327149 at 1 : 5000–1 : 10000 dilution, showing strong cytoplasmic positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human cerebral cortex using ab327149 at 1 : 5000–1 : 10000 dilution, showing moderate membranous positivity in a subset of neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human colorectal cancer using ab327149 at 1 : 5000–1 : 10000 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human ovarian cancer (dysgeminoma) using ab327149 at 1 : 5000–1 : 10000 dilution, showing strong membranous and cytoplasmic positivity in tumor cells.

Supplier Data

Western blot - Anti-LGR5 Antibody [CL13567] (AB327149)

Western blot analysis in human cell line SH-SY5Y using ab327149 at 1 µg/ml.

All lanes:

Western blot - Anti-LGR5 Antibody [CL13567] (ab327149)

All lanes:

Human cell line SH-SY5Y

Predicted band size: 100 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13567] (AB327149)

Immunohistochemical staining of human tonsil using ab327149 at 1 : 5000–1 : 10000 dilution, showing no positivity in non-germinal center cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13567

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LGR5.

O75473

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

See full target information LGR5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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