AB327150

Anti-LGR5 Antibody [CL13579]

Name: Anti-LGR5 Antibody [CL13579] (ab327150) | Abcam
Brand: Abcam Limited
SKU: AB327150
Price: 580 USD
Availability: InStock

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Mouse Monoclonal LGR5 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LGR5.

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human ovarian cancer (papillary carcinoma) using ab327150 at 1 : 200–1 : 500 dilution, showing moderate to strong cytoplasmic and memrbanous positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human tonsil using ab327150 at 1 : 200–1 : 500 dilution, showing mainly very weak positivity in non-germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human cerebral cortex using ab327150 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic and membranous positivity in neurons and neuropil.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemistry analysis in human placenta and tonsil tissues using ab327150 at 1 : 200–1 : 500 dilution. Corresponding LGR5 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human colorectal cancer using ab327150 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human placenta using ab327150 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic and membranous positivity in trophoblastic cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human ovarian cancer (endometrioid carcinoma) using ab327150 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 Antibody [CL13579] (AB327150)

Immunohistochemical staining of human duodenum using ab327150 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic and membranous positivity in glandular cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13579

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human LGR5.

O75473

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

See full target information LGR5

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Product promise

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