AB75850

Anti-LGR5 antibody [EPR3065Y]

Name: Anti-LGR5 antibody [EPR3065Y] (ab75850) | Abcam
Brand: Abcam Limited
SKU: AB75850
Price: 405 USD
Availability: InStock
Rating: 4 (7 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(7 Reviews)

(74 Publications)

Anti-LGR5 antibody [EPR3065Y] (ab75850) is a rabbit monoclonal antibody detecting LGR5 in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications
- Trusted since 2009

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

3 Images

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Supplier Data

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

We are unsure about the identity of the additional band.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate at 20 µg

Lane 2:

Rat skeletal muscle lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution

All lanes:

Fetal skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Carrier free

Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3065Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-LGR5 antibody [EPR3065Y] (ab75850) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of LGR5?
Anti-LGR5 [EPR3065Y] (ab75850) specifically detects a band for LGR5 (UniProt: O75473) at a molecular weight of 100kDa.

Trusted by the scientific community
Anti-LGR5 [EPR3065Y] (ab75850) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-LGR5 [EPR3065Y] (ab75850) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3065Y] also available for your convenience: ab75850, Carrier free - ab199335

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.

Biological function summary

LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.

Pathways

LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as Β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.

LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

See full target information LGR5

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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 52:995 PubMed41065962

2025

R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Hu,Yoshitaka Mita,Xinhe Zhu,Yasuro Furuichi,Yoshimi Nakagawa,Yasuko Manabe,Nobuharu L Fujii

Nature communications 16:8598 PubMed41022821

2025

Cytoplasmic TRIM24 promotes colorectal cancer cell proliferation by activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Wang,Yuanbing Yao,Zehong Liu,Shichao Long,Feng Yi,Qing Fang,Dongbo Wu,Qing Zhu,Hongyan Zai,Shuai Xiao,Fengyi Wan,Kai Fu

Biomolecules & therapeutics 33:842-851 PubMed40765264

2025

An Endolichenic Fungi-Derived Fatty Acid, cis-10-Nonadecenoic acid, Suppresses Colorectal Cancer Stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Mücahit Varlı,Eun-Young Lee,So-Yeon Park,Yi Yang,Prima F Hillman,Rui Zhou,Jae-Seoun Hur,Sang-Jip Nam,Hangun Kim

Materials today. Bio 32:101700 PubMed40225139

2025

Engineering a dynamic extracellular matrix using thrombospondin-1 to propel hepatocyte organoids reprogramming and improve mouse liver regeneration post-transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yan Xu,Min Wang,Jing-Yan Shi,Ye Liu,Chao Yu,Xin-Yi Zhang,Chen-Wei Zhang,Qi-Feng He,Chao Pan,Jin Zhou,Hua Xiao,Hong-Yong Cao,Yong Ma

Clinical and translational medicine 15:e70152 PubMed39748192

2025

Exosome-derived long non-coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Chu,Lingling Jia,Yulong Li,Jiachao Xiong,Yulin Sun,Qin Zhou,Dexiang Du,Zihan Li,Xin Huang,Hua Jiang,Baojin Wu,Yufei Li

Life science alliance 7: PubMed39191487

2024

induces the expression of Lgr5 and stem cell properties in gastric target cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zuzana Nascakova,Jiazhuo He,Giovanni Papa,Biel Francas,Flora Azizi,Anne Müller

Virulence 15:2384553 PubMed39080852

2024

promotes activation of intestinal ILC3 through TLR2 receptor and inhibits infection in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junhong Wang,Ming Gao,Jiarui Wang,Yan Zeng,Chunfeng Wang,Xin Cao

Oncology letters 28:420 PubMed39006950

2024

Poorly cohesive gastric cancer with increased epithelial‑mesenchymal transition is associated with a poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhiro Nakazawa,Makoto Sohda,Munenori Ide,Yuki Shimoda,Akihiko Sano,Makoto Sakai,Tetsunari Oyama,Ken Shirabe,Hiroshi Saeki

Aging 16:9410-9436 PubMed38848145

2024

APOA5 alleviates reactive oxygen species to promote oxaliplatin resistance in PIK3CA-mutated colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Lin Liu,Zhuo Xiang,Bo-Ya Zhang,Yu-Wei Zou,Gui-Lai Chen,Li Yin,Yan-Long Shi,Li-Li Xu,Jingwang Bi,Qiang Wang

Heliyon 10:e30330 PubMed38726177

2024

NAT10-mediated acC modification promotes stemness and chemoresistance of colon cancer by stabilizing NANOGP8.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Gao,Ting-Dong Li,Su-Zhen Yang,Hui-Min Ma,Xiang Wang,De-Kui Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGR5 antibody [EPR3065Y]

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (74)

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