AB199335

Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal LGR5 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

View Alternative Names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

3 Images

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/en-us/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Supplier Data

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

We are unsure about the identity of the additional band.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/en-us/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate at 20 µg

Lane 2:

Rat skeletal muscle lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/en-us/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

All lanes:

Fetal skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Unconjugated

Anti-LGR5 antibody [EPR3065Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3065Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab199335 is the carrier-free version of ab75850.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.

Biological function summary

LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.

Pathways

LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.

LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

See full target information LGR5

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7808 PubMed40841558

2025

Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Miesje M van der Stoel,Shreyas Kaptan,Haoran Li,Shiqian Li,Maarit Hölttä,Helena Vihinen,Eija Jokitalo,Christoph Thiele,Olli Pietiläinen,Shin Morioka,Junko Sasaki,Takehiko Sasaki,Ilpo Vattulainen,Elina Ikonen

Frontiers in oncology 12:805290 PubMed35299743

2022

Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Zhang,Lu Kong,Hongbin Zhu,Decong Sun,Quanli Han,Bin Yan,Zhi Cui,Weiwei Zhang,Shurong Zhang,Xindan Kang,Guanghai Dai,Niansong Qian,Wenji Yan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise