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Anti-LGR5 antibody OTI2A2 is a mouse monoclonal antibody that is used to detect LGR5 in ICC/IF, Flow cytometry. Suitable for Human samples.


Images

Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092), expandable thumbnail
  • Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (AB273092), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human LGR5 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link O75473

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested
Recombinant fragment
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Alternative names

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

Recommended products

Anti-LGR5 antibody OTI2A2 is a mouse monoclonal antibody that is used to detect LGR5 in ICC/IF, Flow cytometry. Suitable for Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human LGR5 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link O75473
Clone number
OTI2A2
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-LGR5 antibody [OTI2A2] (ab273092) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF in human samples.
Anti-LGR5 antibody [OTI2A2] (ab273092) specifically detects LGR5 (UniProt ID: O75473; Molecular weight: 98kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-LGR5 antibody [OTI2A2] (ab273092) has high sensitivity and specificity.



Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LGR5, labeling LGR5 using ab273092 at 1/100 dilution.

  • Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092), expandable thumbnail

    Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Flow cytometric analysis of HEK-293T cells transfected with either LGR5 overexpressed plasmid (Red) or empty vector control plasmid (Blue). Cells were immunostained with ab273092 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunofluorescent staining of HT-29 cells labeling LGR5 using ab273092 at 1/100 dilution (green). Actin filaments were labeled with TRITC-phalloidin (red). The nuclear counter stain is DAPI (blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com