Rabbit Polyclonal LH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLOD1 aa 300-400.
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- Laboratory investigation; a journal of technical methods and pathology 101:564-5692023PLODs are overexpressed in ovarian cancer and are associated with gap junctions via connexin 43.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Guo,Chao Gu,Bin Li,Congjian XuPubMed 36775379
- Theranostics 11:9587-96042021Lysyl hydroxylase 1 (LH1) deficiency promotes angiotensin II (Ang II)-induced dissecting abdominal aortic aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Li,Haochen Xu,Hongyan Wen,Hongyue Wang,Ranxu Zhao,Yingying Sun,Congxia Bai,Jiedan Ping,Li Song,Mingyao Luo,Jingzhou ChenPubMed 34646388
- Laboratory investigation; a journal of technical m :2021PLODs are overexpressed in ovarian cancer and are associated with gap junctions via connexin 43.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Guo et. al.PubMed 33483598
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLOD1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q02809
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:10686424, PubMed:15854030, PubMed:8621606). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Alternative names
LLH, PLOD, PLOD1, Lysyl hydroxylase 1, LH1
Recommended products
Rabbit Polyclonal LH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLOD1 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLOD1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q02809
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LH1 also known as Luteinizing Hormone subunit beta plays an important mechanical role in reproductive biology. The molecular mass of LH1 is approximately 15 kDa. It is chiefly expressed in the anterior pituitary gland. This expression is essential for mediating reproductive processes by interacting with other hormones and receptors located in various tissues including the gonads. The activity of LH1 involves binding and signaling through specific hormone receptors therefore instigating a cascade of biological responses.
- Biological function summary
LH1 engages in regulatory functions within the reproductive system facilitating ovulation and stimulating the production of sex steroids such as testosterone and estradiol. LH1 operates as part of a dimeric glycoprotein hormone complex which includes the alpha and beta subunits that also construct other glycoprotein hormones like TSH and FSH. This complex plays a fundamental role in orchestrating the hormonal milieu necessary for reproductive organ function and development.
- Pathways
LH1 operates within the hypothalamic-pituitary-gonadal (HPG) axis. This pathway involves a series of hormonal signals starting from GnRH release from the hypothalamus prompting the synthesis and release of LH1 from the pituitary. Within this axis LH1 works in conjunction with related proteins like FSH to regulate reproductive functions. These pathways ensure proper feedback regulation of hormone levels important for maintaining reproductive health and function.
- Associated diseases and disorders
LH1 abnormalities have associations with reproductive conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS an imbalance in LH1 and FSH levels can disrupt normal ovarian function leading to symptoms like irregular menstruation and infertility. In hypogonadism LH1 levels may be insufficient to stimulate normal gonadal activity. Prolactin another hormone interacting in these disorders can also influence the functioning of LH1 adding complexity to the underlying pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LH1 antibody (ab262947)
Western blot analysis using ab262947 shows a similar staining pattern as an independent antibody (B).
All lanes: Western blot - Anti-LH1 antibody (ab262947) at 0.4 µg/mL
Lane 1: RT4 cell lysate
Lane 2: U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3: Human plasma
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LH1 antibody (ab262947)
Paraffin-embedded Human placenta tissue stained for LH1 using ab262947 at 1/50 dilution in immunohistochemical analysis.
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