Rabbit Recombinant Monoclonal Luteinizing Hormone beta antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-LHB antibody [EPR19257-172] - BSA and Azide free (Detector) (AB245049), expandable thumbnail](https://content.abcam.com/products/images/lhb-antibody-epr19257-172-bsa-and-azide-free-detector-ab245049--sandwich-elisa-img80144.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-LHB antibody [EPR19257-172] - BSA and Azide free (Detector) (AB245049)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Alternative names
Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, Lhb
Recommended products
Rabbit Recombinant Monoclonal Luteinizing Hormone beta antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19257-172
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab245049 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse LH Beta. The recommended pair for sandwich ELISA is:
Capture: Anti-LHB antibody [EPR19257-177] - BSA and Azide free (Capture) ab244776, Mouse LH Beta Capture Antibody (unconjugated)
Detector: ab245049, Mouse LH Beta Detector Antibody (unconjugated)
The reference range value is 117 - 7500 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.
- Biological function summary
LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.
- Pathways
The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.
- Associated diseases and disorders
Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-LHB antibody [EPR19257-172] - BSA and Azide free (Detector) (ab245049), expandable thumbnail](https://content.abcam.com/products/images/lhb-antibody-epr19257-172-bsa-and-azide-free-detector-ab245049--sandwich-elisa-img80144.jpg "Sandwich ELISA - Anti-LHB antibody [EPR19257-172] - BSA and Azide free (Detector) (ab245049)")
Sandwich ELISA - Anti-LHB antibody [EPR19257-172] - BSA and Azide free (Detector) (ab245049)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse LH Beta ELISA Kit ab235648).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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