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Rabbit Recombinant Monoclonal LHFPL5 antibody. C-terminal. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242), expandable thumbnail
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242), expandable thumbnail
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242), expandable thumbnail
  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/60
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/180
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Auxiliary subunit of the mechanotransducer (MET) non-specific cation channel complex located at the tips of the shorter stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system. The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15. Functionally couples PCDH15 to the transduction channel.

Alternative names

TMHS, LHFPL5, LHFPL tetraspan subfamily member 5 protein, Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia

Recommended products

Rabbit Recombinant Monoclonal LHFPL5 antibody. C-terminal. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16285
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LHFPL5 also known as TMHS (Tetraspan Membrane Protein of the Hair Cell Stereocilia) functions mechanically as a component of mechanotransduction in hair cells. This protein has an estimated mass of around 25 kDa and is mainly expressed in the inner ear's cochlear and vestibular systems. It resides in the stereocilia of hair cells where it plays an important role in auditory systems through its association with mechanotransduction channels.

Biological function summary

LHFPL5 contributes to auditory transduction by interacting with other proteins in a complex critical for hair cell function. This complex is involved in converting mechanical stimuli into neural signals. LHFPL5 supports the integrity and function of the mechanotransduction channel allowing the inner ear to detect sound and balance changes. The protein acts as a molecular anchor directly influencing the gating of the channel and overall sensory capabilities.

Pathways

LHFPL5 integrates into the auditory mechanotransduction pathway necessary for hearing. It works in conjunction with proteins such as protocadherin 15 (PCDH15) and myosin 7a which are vital for the linking of molecular structures within the stereocilia. These interactions contribute to the pathway's role in enabling the propagation of electrical signals generated from mechanical excitation fundamental to auditory perception.

Associated diseases and disorders

Mutations in LHFPL5 are linked to Usher syndrome which causes deafness and progressive vision loss. Additionally its dysfunction is associated with non-syndromic hearing loss. These conditions often involve abnormal interactions with other implicated proteins such as cadherin 23 (CDH23) highlighting the interconnected nature of LHFPL5 within the structural and functional frameworks essential for normal hearing and balance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242), expandable thumbnail

    Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    Predicted band size: 24 kDa

  • Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling LHFPL5with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242), expandable thumbnail

    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    All lanes: Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Rat heart lysate at 10 µg

    Lane 5: Rat kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242), expandable thumbnail

    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    All lanes: Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution

    All lanes: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242), expandable thumbnail

    Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

    All lanes: Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: Human fetal lung lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 24 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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