AB192242

Anti-LHFPL5 antibody [EPR16285] - C-terminal

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal LHFPL5 antibody. C-terminal. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

TMHS, LHFPL5, LHFPL tetraspan subfamily member 5 protein, Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia

5 Images

Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling LHFPL5with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

All lanes:

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

All lanes:

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

All lanes:

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 24 kDa

false

Carrier free

Anti-LHFPL5 antibody [EPR16285] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16285

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/180", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LHFPL5 also known as TMHS (Tetraspan Membrane Protein of the Hair Cell Stereocilia) functions mechanically as a component of mechanotransduction in hair cells. This protein has an estimated mass of around 25 kDa and is mainly expressed in the inner ear's cochlear and vestibular systems. It resides in the stereocilia of hair cells where it plays an important role in auditory systems through its association with mechanotransduction channels.

Biological function summary

LHFPL5 contributes to auditory transduction by interacting with other proteins in a complex critical for hair cell function. This complex is involved in converting mechanical stimuli into neural signals. LHFPL5 supports the integrity and function of the mechanotransduction channel allowing the inner ear to detect sound and balance changes. The protein acts as a molecular anchor directly influencing the gating of the channel and overall sensory capabilities.

Pathways

LHFPL5 integrates into the auditory mechanotransduction pathway necessary for hearing. It works in conjunction with proteins such as protocadherin 15 (PCDH15) and myosin 7a which are vital for the linking of molecular structures within the stereocilia. These interactions contribute to the pathway's role in enabling the propagation of electrical signals generated from mechanical excitation fundamental to auditory perception.

Mutations in LHFPL5 are linked to Usher syndrome which causes deafness and progressive vision loss. Additionally its dysfunction is associated with non-syndromic hearing loss. These conditions often involve abnormal interactions with other implicated proteins such as cadherin 23 (CDH23) highlighting the interconnected nature of LHFPL5 within the structural and functional frameworks essential for normal hearing and balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Auxiliary subunit of the mechanotransducer (MET) non-specific cation channel complex located at the tips of the shorter stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system. The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15. Functionally couples PCDH15 to the transduction channel.

See full target information LHFPL5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:3331-3343 PubMed33707295

2021

Mutations Cause Mechanotransduction Defects in Cochlear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alix Trouillet,Katharine K Miller,Shefin Sam George,Pei Wang,Noor-E-Seher Ali,Anthony Ricci,Nicolas Grillet

FASEB journal : official publication of the Federa 33:6838-6851 PubMed30808210

2019

Localization of TMC1 and LHFPL5 in auditory hair cells in neonatal and adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofen Li,Xiaojie Yu,Xibing Chen,Zhengzhao Liu,Guangqin Wang,Chao Li,Elaine Y M Wong,Mai Har Sham,Jie Tang,Jufang He,Wei Xiong,Zhiyong Liu,Pingbo Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LHFPL5 antibody [EPR16285] - C-terminal

Flow Cytometry (Intracellular) - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (AB192242)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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