AB254788

Anti-LHPP antibody

Name: Anti-LHPP antibody (ab254788) | Abcam
Brand: Abcam Limited
SKU: AB254788
Price: 655 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal LHPP antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase aa 150 to C-terminus.

View Alternative Names

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, LHPP

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-LHPP antibody (AB254788)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LHPP antibody (AB254788)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling LHPP using ab254788 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Paraffin-embedded human skeletal muscle tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Paraffin-embedded human kidney tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Paraffin-embedded human liver tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Paraffin-embedded human cerebral cortex tissue stained for LHPP using ab254788 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LHPP antibody (AB254788)

Western blot analysis labeling LHPP with ab254788 at 0.04 ug/ml.

All lanes:

Western blot - Anti-LHPP antibody (ab254788) at 0.04 µg/mL

All lanes:

Cerebral cortex tissue lysate

Predicted band size: 29 kDa

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Supplier Data

Western blot - Anti-LHPP antibody (AB254788)

All lanes:

Western blot - Anti-LHPP antibody (ab254788) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H008

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LHPP protein also known as phospholysine phosphohistidine inorganic pyrophosphate phosphatase serves as a histidine phosphatase. It has a molecular mass of approximately 29 kDa and is expressed in various tissues including the liver and brain. The protein catalyzes the hydrolysis of phosphoramidate bonds in specific substrates playing a role in cellular phosphate metabolism.

Biological function summary

LHPP acts on a complex network of phosphorylated molecules. This protein regulates intracellular phosphate levels impacting processes like energy production and signal transduction. LHPP is essential in controlling phosphorylation status affecting cellular functions such as growth and differentiation by interacting with substrates within the cell.

Pathways

LHPP participates in key biochemical routes like the phosphatidylinositol signaling pathway and possibly glycolysis. Within these pathways LHPP affects the activity of proteins like phosphatidylinositol-45-bisphosphate 3-kinase (PI3K) influencing cellular decision-making processes related to metabolism and survival. Understanding LHPP interactions aids in deciphering signal relay and metabolic adjustments in cells.

Alterations in LHPP expression associate with liver diseases and neurological disorders. In liver diseases an abnormality in phosphate metabolism could contribute to disease progression. In neurological contexts changes in LHPP or its pathway components may relate to diseases like epilepsy possibly through interactions with proteins affecting neuronal excitability such as voltage-gated ion channels. Connection of LHPP to these proteins highlights its significance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency (By similarity).

See full target information Phospholysine phosphohistidine inorganic pyrophosphate phosphatase

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1463673 PubMed39902076

2025

α-methyltryptophan-mediated protection against diabetic nephropathy in mice as studied with a metabolomics approach.

Applications

Unspecified application

Species

Unspecified reactive species

Aimin Cai,Dingchao Shen,Qiushuang Xiong,Jie Ding,Yang Ding,Xinlu Lin,Lijia Chen,Qing Yao,Guangyong Lin,Ruijie Chen,Vadivel Ganapathy,Longfa Kou

Technology in cancer research & treatment 23:15330338241293485 PubMed39497541

2024

Single-Cell Transcriptomics Reveals STAT1 Drives LHPP Downregulation in Esophageal Squamous Cell Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Chen,Zhiyun Xu,Wubi Zhou,Lili Zhang,Jian Huang,Dawei Bao

Heliyon 10:e29163 PubMed38601522

2024

Single-cell transcriptomics reveals activation of endothelial cell and identifies LHPP as a potential target in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyu He,Yanfei Wang,Chen Shuang,Chan Xu,Xiaoling Li,Yanfei Cao

World journal of gastroenterology 29:6222-6234 PubMed38186864

2024

Single-cell analysis identifies phospholysine phosphohistidine inorganic pyrophosphate phosphatase as a target in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Fei Wang,Ruo-Yu He,Chan Xu,Xiao-Ling Li,Yan-Fei Cao

Technology in cancer research & treatment 22:15330338231177807 PubMed37321804

2023

LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaozhi Xia,Shuchao Zhao,Xin Gao,Hongrui Sun,Faji Yang,Huaqiang Zhu,Hengjun Gao,Jun Lu,Xu Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LHPP antibody

Immunocytochemistry/ Immunofluorescence - Anti-LHPP antibody (AB254788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHPP antibody (AB254788)

Western blot - Anti-LHPP antibody (AB254788)

Western blot - Anti-LHPP antibody (AB254788)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise