AB243030

Anti-Lhx2/LH2 antibody [CL6137]

Name: Anti-Lhx2/LH2 antibody [CL6137] (ab243030) | Abcam
Brand: Abcam Limited
SKU: AB243030
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal Lhx2/LH2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human LHX2 aa 200-250.

View Alternative Names

LH2, LHX2, LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

Formalin-fixed, paraffin-embedded mouse embryo E14 tissue stained for Lhx2/LH2 with ab243030 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

All lanes:

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (ab243030) at 1 µg/mL

All lanes:

Mouse cerebral cortex lysate

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

All lanes:

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (ab243030) at 1 µg/mL

All lanes:

Human cerebral cortex lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL6137

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LHX2 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P50458

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lhx2 protein also known as LH2 is a member of the LIM homeobox family of transcription factors. It has a molecular mass of approximately 45 kDa. Known for its dual LIM domains and a homeodomain Lhx2 directly binds DNA and regulates gene expression. This protein is expressed in various tissues including the developing brain retina and liver. Notably Lhx2 plays a role in embryonic development by impacting the growth and differentiation of several tissue types.

Biological function summary

Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.

Pathways

Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.

Mutations or dysregulation of Lhx2 have connections to conditions like anophthalmia and brain malformations. Aberrant expression of Lhx2 can disrupt normal development contributing to these congenital anomalies. Furthermore Lhx2's interaction with proteins including Pax6 plays a role in ocular development and anomalies in these interactions can lead to abnormal eye formation. Researchers continue to investigate the significance of Lhx2 in various developmental diseases to develop targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity).

See full target information LHX2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomacromolecules 25:6485-6502 PubMed39214607

2024

Harnessing the Biomimetic Effect of Macromolecular Crowding in the Cell-Derived Model of Clubfoot Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Doubková,Jarmila Knitlová,David Vondrášek,Adam Eckhardt,Tomáš Novotný,Martin Ošt'ádal,Elena Filová,Lucie Bačáková

British journal of cancer 127:1239-1253 PubMed35864158

2022

LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Xie,Kunpeng Du,Wei Liu,Chunshan Liu,Baiyao Wang,Yunhong Tian,Rong Li,Xiaoting Huang,Jie Lin,Haifeng Jian,Jian Zhang,Yawei Yuan

Bioengineered 13:8208-8225 PubMed35300558

2022

Long non-coding RNA PTPRG-AS1/microRNA-124-3p regulates radiosensitivity of nasopharyngeal carcinoma via the LIM Homeobox 2-dependent Notch pathway through competitive endogenous RNA mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangquan Shen,Yang Wu,Guijun He

Journal of cellular biochemistry 120:15018-15026 PubMed31017720

2019

miR-144 reverses cisplatin resistance in cervical cancer via targeting LHX2.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Shi,Jin Su,Zi Liu,Jiquan Wang,Tao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lhx2/LH2 antibody [CL6137]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

Western blot - Anti-Lhx2/LH2 antibody [CL6137] (AB243030)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise