Anti-Lhx2/LH2 antibody [EPR20449]
- RabMAb
- Recombinant
- 20ul selling size
- What is this?
5
(6 Reviews)
|
(24 Publications)
Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) is a rabbit monoclonal antibody detecting Lhx2/LH2 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
View Alternative Names
LH2, LHX2, LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID : 15456728].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID : 20386748].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID : 25071464].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID : 25071464].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID : 15456728].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID : 15456728].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID : 27521061].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID : 20386748].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Supplier Data
Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : E18 rat brain lysate, 10 μg (Input).
Lane 2 : ab184337 IP in E18 rat brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.
All lanes:
Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
Predicted band size: 44 kDa
Observed band size: 47 kDa
false
- WB
Supplier Data
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Blocking/Dilution buffer : 5% NFDM/TBST.
Molecular weight observed is consistent with what has been described in the literature, PMID 22028024, PMID 25128627.
All lanes:
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/200 dilution
All lanes:
E12.5 mouse brain lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
false
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution
All lanes:
E18 rat brain lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
false
Exposure time: 3min
- WB
Lab
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST
Lanes 1 - 2:
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037'>ab236037</a>) at 1/1000 dilution
Lanes 1 - 2:
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution
Lane 1:
293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
293T transfected with human Lhx2/LH2 (WT) expression vector containing a GFP-tag, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 80 kDa
false
Exposure time: 3s
- WB
Supplier Data
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution
Lane 1:
His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426) at 0.01 µg
Lane 2:
His-tagged rat Lhx9 recombinant protein fragment (aa54-172) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
false
Exposure time: 3min
Related conjugates and formulations (2)
-
Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449]
Reactivity data
Product details
What is this antibody validated in?
Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of Lhx2/LH2?
Anti-Lhx2/LH2 [EPR20449] (ab184337) specifically detects a band for Lhx2/LH2 (UniProt: P36198) at a molecular weight of 44kDa.
Trusted by the scientific community
Anti-Lhx2/LH2 [EPR20449] (ab184337) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-Lhx2/LH2 [EPR20449] (ab184337) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR20449] also available for your convenience: ab184337, Carrier free - ab236037, Alexa Fluor® 647 - ab322021
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.
Pathways
Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like Β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (24)
Recent publications for all applications. Explore the full list and refine your search
Genes & development 39:1338-1354 PubMed40774817
2025
Applications
Unspecified application
Species
Unspecified reactive species
Biology open 14: PubMed40557565
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:5132 PubMed40461535
2025
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of cell biology 224: PubMed39879198
2025
Applications
Unspecified application
Species
Unspecified reactive species
Science advances 10:eadi5791 PubMed38241368
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature : PubMed38057666
2023
Applications
Unspecified application
Species
Unspecified reactive species
Developmental cell 58:2652-2665.e6 PubMed37683631
2023
Applications
Unspecified application
Species
Unspecified reactive species
Stem cells (Dayton, Ohio) 41:1037-1046 PubMed37632456
2023
Applications
Unspecified application
Species
Unspecified reactive species
International heart journal 64:750-758 PubMed37518356
2023
Applications
Unspecified application
Species
Unspecified reactive species
International journal of biological sciences 19:3226-3248 PubMed37416774
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com