Rabbit Recombinant Monoclonal Lhx2/LH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108784.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)")
![Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunoprecipitation-img55694.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)")
![Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-frozen-sections-img113287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115196.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23291.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Predicted | Expected | Predicted | Predicted |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Tested | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity).
Alternative names
LH2, LHX2, LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2
Recommended products
Rabbit Recombinant Monoclonal Lhx2/LH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20449
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236037 is the carrier-free version of Anti-Lhx2/LH2 antibody [EPR20449] ab184337.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Lhx2 protein also known as LH2 is a member of the LIM homeobox family of transcription factors. It has a molecular mass of approximately 45 kDa. Known for its dual LIM domains and a homeodomain Lhx2 directly binds DNA and regulates gene expression. This protein is expressed in various tissues including the developing brain retina and liver. Notably Lhx2 plays a role in embryonic development by impacting the growth and differentiation of several tissue types.
- Biological function summary
Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.
- Pathways
Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.
- Associated diseases and disorders
Mutations or dysregulation of Lhx2 have connections to conditions like anophthalmia and brain malformations. Aberrant expression of Lhx2 can disrupt normal development contributing to these congenital anomalies. Furthermore Lhx2's interaction with proteins including Pax6 plays a role in ocular development and anomalies in these interactions can lead to abnormal eye formation. Researchers continue to investigate the significance of Lhx2 in various developmental diseases to develop targeted therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108784.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID: 15456728].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunoprecipitation-img55694.jpg "Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: E18 rat brain lysate, 10 μg (Input).
Lane 2: Anti-Lhx2/LH2 antibody [EPR20449] ab184337 IP in E18 rat brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Lhx2/LH2 antibody [EPR20449] ab184337 in E18 rat brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
All lanes: Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (Anti-Lhx2/LH2 antibody [EPR20449] ab184337)
Predicted band size: 44 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-frozen-sections-img113287.jpg "Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID: 27521061].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115196.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID: 25071464].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23291.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID: 15456728].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-frozen-sections-img119192.jpg "Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID: 15456728].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48036.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID: 25071464].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111212.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID: 20386748].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with Anti-Lhx2/LH2 antibody [EPR20449] ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID: 20386748].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lhx2/LH2 antibody [EPR20449] ab184337).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--western-blot-img400042.jpg "Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
This data was developed using Anti-Lhx2/LH2 antibody [EPR20449] ab184337, the same antibody clone in a different buffer formulation.Lanes 1 - 2: Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (Anti-Lhx2/LH2 antibody [EPR20449] ab184337) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T transfected with human Lhx2/LH2 (WT) expression vector containing a GFP-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 80 kDa
Exposure time: 3s
![Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-frozen-sections-img119192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48036.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111212.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
![Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037), expandable thumbnail](https://content.abcam.com/products/images/lhx2-lh2-antibody-epr20449-bsa-and-azide-free-ab236037--western-blot-img400042.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)")
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