AB236037

Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Lhx2/LH2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

LH2, LHX2, LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID : 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID : 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID : 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID : 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID : 15456728].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID : 27521061].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID : 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID : 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : E18 rat brain lysate, 10 μg (Input).

Lane 2 : ab184337 IP in E18 rat brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184337).

All lanes:

Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (<a href='/en-us/products/primary-antibodies/lhx2-lh2-antibody-epr20449-ab184337'>ab184337</a>)

Predicted band size: 44 kDa

Observed band size: 47 kDa

false

Lab

Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This data was developed using ab184337, the same antibody clone in a different buffer formulation.

Lanes 1 - 2:

Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (<a href='/en-us/products/primary-antibodies/lhx2-lh2-antibody-epr20449-ab184337'>ab184337</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T transfected with human Lhx2/LH2 (WT) expression vector containing a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 80 kDa

false

Exposure time: 3s

Unconjugated

Anti-Lhx2/LH2 antibody [EPR20449]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Lhx2/LH2 antibody [EPR20449]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20449

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab236037 is the carrier-free version of ab184337.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lhx2 protein also known as LH2 is a member of the LIM homeobox family of transcription factors. It has a molecular mass of approximately 45 kDa. Known for its dual LIM domains and a homeodomain Lhx2 directly binds DNA and regulates gene expression. This protein is expressed in various tissues including the developing brain retina and liver. Notably Lhx2 plays a role in embryonic development by impacting the growth and differentiation of several tissue types.

Biological function summary

Researchers link the Lhx2 protein to key processes in cellular differentiation and organ development. Lhx2 often partners within transcriptional complexes that guide cellular fate decisions. By interacting with other cofactors Lhx2 influences the expression of genes involved in tissue-specific differentiation and morphogenesis. This regulatory action is essential for the proper formation and function of certain organs and structures in the body.

Pathways

Lhx2 impacts several intricate biological pathways notably in neural development. It functions within the Wnt signaling pathway where it modulates neural progenitor cell populations in the central nervous system. Through this pathway Lhx2 associates with proteins like β-catenin to control gene expression linked to brain development. Additionally Lhx2 is involved in the Sonic hedgehog (Shh) signaling pathway aligning with proteins such as Gli1 to influence neuronal patterning.

Mutations or dysregulation of Lhx2 have connections to conditions like anophthalmia and brain malformations. Aberrant expression of Lhx2 can disrupt normal development contributing to these congenital anomalies. Furthermore Lhx2's interaction with proteins including Pax6 plays a role in ocular development and anomalies in these interactions can lead to abnormal eye formation. Researchers continue to investigate the significance of Lhx2 in various developmental diseases to develop targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity).

See full target information LHX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC pharmacology & toxicology 23:81 PubMed36273189

2022

Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Zhang,Xiue Mu,Xiaohui Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Western blot - Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (AB236037)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise