AB124697

Anti-LHX3/LIM antibody [EPR3344(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal LHX3/LIM antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

LIM/homeobox protein Lhx3, LIM homeobox protein 3, LHX3

3 Images

Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

ab124697 at 1/10 dilution, staining LHX3/LIM in permeabilized Jurkat cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

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Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

All lanes:

Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697) at 1/1000 dilution

Lane 1:

293T(Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

Human brain tissue lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

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OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

OI-RD Scanning

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OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3344(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LHX3 also known as LIM homeobox 3 protein is a transcription factor involved in the development and differentiation of specific tissues in the human body. It is expressed mainly in the pituitary gland forebrain and spinal cord. The molecular weight of LHX3 is approximately 45 kDa. This transcription factor contains two LIM domains and one homeodomain which are significant for its DNA-binding capability. Through these domains LHX3 regulates the expression of target genes critical for tissue-specific development.

Biological function summary

LHX3 is essential for the proper formation of the pituitary gland and motor neurons. It acts as part of a complex that includes other LIM homeodomain proteins to modulate transcription of genes responsible for lineage specification. LHX3 functions by binding to DNA sequences and affecting the transcriptional activation or repression of gene targets influencing processes like hormone biosynthesis in the pituitary. This regulation ensures appropriate cellular responses during development and differentiation.

Pathways

LHX3 interacts within the Wnt and Notch signaling pathways which are pivotal in developmental processes. In the Wnt pathway it modulates gene expression that governs cell proliferation and differentiation. The Notch pathway involvement highlights its role in neural differentiation. Through these pathways LHX3 works closely with proteins like β-catenin in the Wnt pathway impacting how cells communicate and respond to developmental cues.

LHX3 mutations are associated with Combined Pituitary Hormone Deficiency (CPHD) and pituitary hormone synthesis issues resulting in growth and developmental disorders. LHX3 defects can interact with other proteins like PIT1 influencing the clinical presentation of CPHD. The understanding of LHX3's role in these pathways and its interaction with proteins offers insight into therapeutic approaches to mitigate these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor. Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2. Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system (PubMed : 21149718). Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (By similarity).

See full target information LHX3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 39: PubMed30626726

2019

HOXD8/DIAPH2-AS1 epigenetically regulates PAX3 and impairs HTR-8/SVneo cell function under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Feng,Jianxia Wang,Yue He,Heng Zhang,Minhui Jiang,Dandan Cao,Aiping Wang

Nature communications 2:214 PubMed21364553

2011

Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state.

Applications

Unspecified application

Species

Unspecified reactive species

R Patani,A J Hollins,T M Wishart,C A Puddifoot,S Alvarez,A R de Lera,D J A Wyllie,D A S Compston,R A Pedersen,T H Gillingwater,G E Hardingham,N D Allen,S Chandran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LHX3/LIM antibody [EPR3344(2)]

Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise