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Rabbit Recombinant Monoclonal LHX3/LIM antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.

Alternative names

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Rabbit Recombinant Monoclonal LHX3/LIM antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3344(2)

Purification technique

Affinity purification Protein A

Dissociation constant

6.84 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab247966 is the carrier-free version of ab124697.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

LHX3 is essential for the proper formation of the pituitary gland and motor neurons. It acts as part of a complex that includes other LIM homeodomain proteins to modulate transcription of genes responsible for lineage specification. LHX3 functions by binding to DNA sequences and affecting the transcriptional activation or repression of gene targets influencing processes like hormone biosynthesis in the pituitary. This regulation ensures appropriate cellular responses during development and differentiation.

Activity summary

LHX3 also known as LIM homeobox 3 protein is a transcription factor involved in the development and differentiation of specific tissues in the human body. It is expressed mainly in the pituitary gland forebrain and spinal cord. The molecular weight of LHX3 is approximately 45 kDa. This transcription factor contains two LIM domains and one homeodomain which are significant for its DNA-binding capability. Through these domains LHX3 regulates the expression of target genes critical for tissue-specific development.

Pathways

LHX3 interacts within the Wnt and Notch signaling pathways which are pivotal in developmental processes. In the Wnt pathway it modulates gene expression that governs cell proliferation and differentiation. The Notch pathway involvement highlights its role in neural differentiation. Through these pathways LHX3 works closely with proteins like β-catenin in the Wnt pathway impacting how cells communicate and respond to developmental cues.

Associated diseases and disorders

LHX3 mutations are associated with Combined Pituitary Hormone Deficiency (CPHD) and pituitary hormone synthesis issues resulting in growth and developmental disorders. LHX3 defects can interact with other proteins like PIT1 influencing the clinical presentation of CPHD. The understanding of LHX3's role in these pathways and its interaction with proteins offers insight into therapeutic approaches to mitigate these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966), expandable thumbnail

    Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966)

    This data was developed using ab124697, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (AB124697) at 1/1000 dilution

    Lane 1: 293T(Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 2: Human brain tissue lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Human fetal lung lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 43 kDa

  • Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966)

    This data was developed using ab124697, the same antibody clone in a different buffer formulation.

    ab124697 at 1/10 dilution, staining LHX3/LIM in permeabilized Jurkat cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

  • OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966), expandable thumbnail

    OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (ab247966)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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