AB247966

Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal LHX3/LIM antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LIM/homeobox protein Lhx3, LIM homeobox protein 3, LHX3

3 Images

Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

This data was developed using ab124697, the same antibody clone in a different buffer formulation.

ab124697 at 1/10 dilution, staining LHX3/LIM in permeabilized Jurkat cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

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Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

This data was developed using ab124697, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (<a href='/en-us/products/primary-antibodies/lhx3-lim-antibody-epr33442-ab124697'>ab124697</a>) at 1/1000 dilution

Lane 1:

293T(Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

Human brain tissue lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

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OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

OI-RD Scanning

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OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-LHX3/LIM antibody [EPR3344(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3344(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab247966 is the carrier-free version of ab124697.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LHX3 also known as LIM homeobox 3 protein is a transcription factor involved in the development and differentiation of specific tissues in the human body. It is expressed mainly in the pituitary gland forebrain and spinal cord. The molecular weight of LHX3 is approximately 45 kDa. This transcription factor contains two LIM domains and one homeodomain which are significant for its DNA-binding capability. Through these domains LHX3 regulates the expression of target genes critical for tissue-specific development.

Biological function summary

LHX3 is essential for the proper formation of the pituitary gland and motor neurons. It acts as part of a complex that includes other LIM homeodomain proteins to modulate transcription of genes responsible for lineage specification. LHX3 functions by binding to DNA sequences and affecting the transcriptional activation or repression of gene targets influencing processes like hormone biosynthesis in the pituitary. This regulation ensures appropriate cellular responses during development and differentiation.

Pathways

LHX3 interacts within the Wnt and Notch signaling pathways which are pivotal in developmental processes. In the Wnt pathway it modulates gene expression that governs cell proliferation and differentiation. The Notch pathway involvement highlights its role in neural differentiation. Through these pathways LHX3 works closely with proteins like β-catenin in the Wnt pathway impacting how cells communicate and respond to developmental cues.

LHX3 mutations are associated with Combined Pituitary Hormone Deficiency (CPHD) and pituitary hormone synthesis issues resulting in growth and developmental disorders. LHX3 defects can interact with other proteins like PIT1 influencing the clinical presentation of CPHD. The understanding of LHX3's role in these pathways and its interaction with proteins offers insight into therapeutic approaches to mitigate these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor. Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription; transcription can be co-activated by LDB2. Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system (PubMed : 21149718). Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (By similarity).

See full target information LHX3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6009 PubMed40593805

2025

Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.

Applications

Unspecified application

Species

Unspecified reactive species

David Smith,Anna Eichinger,Éanna Fennell,Zijun Y Xu-Monette,Andrew Rech,Julia Wang,Eduardo Esteva,Arta Seyedian,Xiaoxu Yang,Mei Zhang,Dan Martinez,Kai Tan,Minjie Luo,Katherine J Young,Paul G Murray,Christopher Park,Boris Reizis,Vinodh Pillai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] - BSA and Azide free (AB247966)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise