Rabbit Polyclonal LHX8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus.
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Publications
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- eLife 12:2023Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Wang,Jing He,Xiaoyu Feng,Shengyou Liao,Yi Zhao,Fuchou Tang,Kehkooi KeePubMed 36602025
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q68G74
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Transcription factor involved in differentiation of certain neurons and mesenchymal cells.
Alternative names
LIM/homeobox protein Lhx8, LIM homeobox protein 8, LHX8
Recommended products
Rabbit Polyclonal LHX8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q68G74
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lhx8 known also as LIM homeobox 8 functions as a transcription factor with a molecular weight of approximately 31 kDa. This protein is part of the LIM homeobox family characterized by the presence of LIM and homeodomain motifs. Lhx8 is mainly expressed in the nervous system and reproductive organs. Expression of Lhx8 has been observed in neuronal tissue specifically influencing neural differentiation and development as well as in the gonads where it plays a role in reproductive system development.
- Biological function summary
Lhx8 regulates the expression of genes involved in the differentiation of specific cell types. It plays an important role in early development and differentiation processes. Lhx8 participates in cell fate decisions directing stem cells towards specific lineages. It does not operate alone but forms complexes with other proteins including Lhx members to exert its effects on cell differentiation particularly in the nervous system and reproductive tissues.
- Pathways
Lhx8 integrates into several important developmental pathways. It is notably active within the retinoic acid signaling pathway which guides cellular differentiation and development. Lhx8 also interacts with Wnt signaling a pathway essential for cell proliferation and fate determination. In these pathways Lhx8 might relate to proteins like β-catenin and other LIM proteins demonstrating its integrative role in facilitating precise developmental processes.
- Associated diseases and disorders
Lhx8 shows relevance in conditions such as infertility and neurodegenerative diseases. Abnormal Lhx8 expression or function has been linked to fertility issues highlighting its involvement in gonadal development. Lhx8's interaction with known neurodegenerative disease-related proteins like those of the retinoic acid pathway indicates its potential connection to neurodegenerative conditions. By understanding Lhx8's role researchers can decipher its contributions to these disorders aiding in potential therapeutic development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Lhx8 antibody (ab221882)
PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for Lhx8 (green) using ab221882 at 4 μg/ml in ICC/IF.
Western blot - Anti-Lhx8 antibody (ab221882)
All lanes: Western blot - Anti-Lhx8 antibody (ab221882) at 1/100 dilution
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 39 kDa
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