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AB221882

Anti-Lhx8 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal LHX8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus.

View Alternative Names

LIM/homeobox protein Lhx8, LIM homeobox protein 8, LHX8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Lhx8 antibody (AB221882)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lhx8 antibody (AB221882)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for Lhx8 (green) using ab221882 at 4 μg/ml in ICC/IF.

Western blot - Anti-Lhx8 antibody (AB221882)
  • WB

Supplier Data

Western blot - Anti-Lhx8 antibody (AB221882)

All lanes:

Western blot - Anti-Lhx8 antibody (ab221882) at 1/100 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LHX8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q68G74

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lhx8 known also as LIM homeobox 8 functions as a transcription factor with a molecular weight of approximately 31 kDa. This protein is part of the LIM homeobox family characterized by the presence of LIM and homeodomain motifs. Lhx8 is mainly expressed in the nervous system and reproductive organs. Expression of Lhx8 has been observed in neuronal tissue specifically influencing neural differentiation and development as well as in the gonads where it plays a role in reproductive system development.
Biological function summary

Lhx8 regulates the expression of genes involved in the differentiation of specific cell types. It plays an important role in early development and differentiation processes. Lhx8 participates in cell fate decisions directing stem cells towards specific lineages. It does not operate alone but forms complexes with other proteins including Lhx members to exert its effects on cell differentiation particularly in the nervous system and reproductive tissues.

Pathways

Lhx8 integrates into several important developmental pathways. It is notably active within the retinoic acid signaling pathway which guides cellular differentiation and development. Lhx8 also interacts with Wnt signaling a pathway essential for cell proliferation and fate determination. In these pathways Lhx8 might relate to proteins like β-catenin and other LIM proteins demonstrating its integrative role in facilitating precise developmental processes.

Lhx8 shows relevance in conditions such as infertility and neurodegenerative diseases. Abnormal Lhx8 expression or function has been linked to fertility issues highlighting its involvement in gonadal development. Lhx8's interaction with known neurodegenerative disease-related proteins like those of the retinoic acid pathway indicates its potential connection to neurodegenerative conditions. By understanding Lhx8's role researchers can decipher its contributions to these disorders aiding in potential therapeutic development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in differentiation of certain neurons and mesenchymal cells.
See full target information LHX8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36602025

2023

Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Jing He,Xiaoyu Feng,Shengyou Liao,Yi Zhao,Fuchou Tang,Kehkooi Kee

Cell reports 34:108670 PubMed33503434

2021

Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfei Jiang,Jane Isquith,Luisa Ladel,Adam Mark,Frida Holm,Cayla Mason,Yudou He,Phoebe Mondala,Isabelle Oliver,Jessica Pham,Wenxue Ma,Eduardo Reynoso,Shawn Ali,Isabella Jamieson Morris,Raymond Diep,Chanond Nasamran,Guorong Xu,Roman Sasik,Sara Brin Rosenthal,Amanda Birmingham,Sanja Coso,Gabriel Pineda,Leslie Crews,Mary E Donohoe,J Craig Venter,Thomas Whisenant,Ruben A Mesa,Ludmil B Alexandrov,Kathleen M Fisch,Catriona Jamieson
View all publications
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Product promise

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