AB137036

Anti-Lhx8 antibody [EPR5213(2)]

Name: Anti-Lhx8 antibody [EPR5213(2)] (ab137036) | Abcam
Brand: Abcam Limited
SKU: AB137036
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal LHX8 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

LIM/homeobox protein Lhx8, LIM homeobox protein 8, LHX8

1 Images

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Western blot - Anti-Lhx8 antibody [EPR5213(2)] (AB137036)

All lanes:

Western blot - Anti-Lhx8 antibody [EPR5213(2)] (ab137036) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal forebrain lysate at 10 µg

Lane 3:

A431 lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 39 kDa

false

Carrier free

Anti-Lhx8 antibody [EPR5213(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5213(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lhx8 known also as LIM homeobox 8 functions as a transcription factor with a molecular weight of approximately 31 kDa. This protein is part of the LIM homeobox family characterized by the presence of LIM and homeodomain motifs. Lhx8 is mainly expressed in the nervous system and reproductive organs. Expression of Lhx8 has been observed in neuronal tissue specifically influencing neural differentiation and development as well as in the gonads where it plays a role in reproductive system development.

Biological function summary

Lhx8 regulates the expression of genes involved in the differentiation of specific cell types. It plays an important role in early development and differentiation processes. Lhx8 participates in cell fate decisions directing stem cells towards specific lineages. It does not operate alone but forms complexes with other proteins including Lhx members to exert its effects on cell differentiation particularly in the nervous system and reproductive tissues.

Pathways

Lhx8 integrates into several important developmental pathways. It is notably active within the retinoic acid signaling pathway which guides cellular differentiation and development. Lhx8 also interacts with Wnt signaling a pathway essential for cell proliferation and fate determination. In these pathways Lhx8 might relate to proteins like β-catenin and other LIM proteins demonstrating its integrative role in facilitating precise developmental processes.

Lhx8 shows relevance in conditions such as infertility and neurodegenerative diseases. Abnormal Lhx8 expression or function has been linked to fertility issues highlighting its involvement in gonadal development. Lhx8's interaction with known neurodegenerative disease-related proteins like those of the retinoic acid pathway indicates its potential connection to neurodegenerative conditions. By understanding Lhx8's role researchers can decipher its contributions to these disorders aiding in potential therapeutic development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in differentiation of certain neurons and mesenchymal cells.

See full target information LHX8

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1394 PubMed41028284

2025

CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Zhang,Yue Zeng,Zhanzhong Qiao,Kexin Qin,Pei Li,Jiamao Yan,Teng Zhang,Yang Zhou,Junjie Wang,Wei Shen

Stem cell research & therapy 12:339 PubMed34112263

2021

Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Delan Huang,Runze Li,Jianhan Ren,Haotian Luo,Weicai Wang,Chen Zhou

Theranostics 11:4992-5009 PubMed33754040

2021

Single-cell transcriptome dissection of the toxic impact of Di (2-ethylhexyl) phthalate on primordial follicle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Wang,Yu Tian,Ming-Hao Li,Yan-Qin Feng,Li Kong,Fa-Li Zhang,Wei Shen

Journal of cellular and molecular medicine 25:3051-3062 PubMed33580754

2021

FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhou,Danying Chen,Jianhan Ren,Delan Huang,Runze Li,Haotian Luo,Chenyu Guan,Yang Cao,Weicai Wang

Cellular physiology and biochemistry : internation 48:556-568 PubMed30021212

2018

Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1α and Mice UCP1.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Hou,Meiying Xie,Lingbo Cao,Jia Shi,Guli Xu,Chingyuan Hu,Chong Wang

Biomaterials 63:35-46 PubMed26081866

2015

Lhx8 mediated Wnt and TGFβ pathways in tooth development and regeneration.

Applications

ChIP, WB

Species

Unspecified reactive species, Unspecified reactive species

Chen Zhou,Guodong Yang,Mo Chen,Chenglin Wang,Ling He,Lusai Xiang,Danying Chen,Junqi Ling,Jeremy J Mao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lhx8 antibody [EPR5213(2)]

Western blot - Anti-Lhx8 antibody [EPR5213(2)] (AB137036)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise