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AB248780

Anti-Lhx8 antibody [EPR5213(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LHX8 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

LIM/homeobox protein Lhx8, LIM homeobox protein 8, LHX8

1 Images
Western blot - Anti-Lhx8 antibody [EPR5213(2)] - BSA and Azide free (AB248780)
  • WB

Unknown

Western blot - Anti-Lhx8 antibody [EPR5213(2)] - BSA and Azide free (AB248780)

This data was developed using ab137036, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Lhx8 antibody [EPR5213(2)] (<a href='/en-us/products/primary-antibodies/lhx8-antibody-epr52132-ab137036'>ab137036</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal forebrain lysate at 10 µg

Lane 3:

A431 lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5213(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248780 is the carrier-free version of ab137036.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lhx8 known also as LIM homeobox 8 functions as a transcription factor with a molecular weight of approximately 31 kDa. This protein is part of the LIM homeobox family characterized by the presence of LIM and homeodomain motifs. Lhx8 is mainly expressed in the nervous system and reproductive organs. Expression of Lhx8 has been observed in neuronal tissue specifically influencing neural differentiation and development as well as in the gonads where it plays a role in reproductive system development.
Biological function summary

Lhx8 regulates the expression of genes involved in the differentiation of specific cell types. It plays an important role in early development and differentiation processes. Lhx8 participates in cell fate decisions directing stem cells towards specific lineages. It does not operate alone but forms complexes with other proteins including Lhx members to exert its effects on cell differentiation particularly in the nervous system and reproductive tissues.

Pathways

Lhx8 integrates into several important developmental pathways. It is notably active within the retinoic acid signaling pathway which guides cellular differentiation and development. Lhx8 also interacts with Wnt signaling a pathway essential for cell proliferation and fate determination. In these pathways Lhx8 might relate to proteins like β-catenin and other LIM proteins demonstrating its integrative role in facilitating precise developmental processes.

Lhx8 shows relevance in conditions such as infertility and neurodegenerative diseases. Abnormal Lhx8 expression or function has been linked to fertility issues highlighting its involvement in gonadal development. Lhx8's interaction with known neurodegenerative disease-related proteins like those of the retinoic acid pathway indicates its potential connection to neurodegenerative conditions. By understanding Lhx8's role researchers can decipher its contributions to these disorders aiding in potential therapeutic development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in differentiation of certain neurons and mesenchymal cells.
See full target information LHX8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com