Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

ab109190 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LI Cadherin antibody [EPR3996] (<a href='/en-us/products/primary-antibodies/li-cadherin-antibody-epr3996-ab109190'>ab109190</a>) at 1/1000 dilution

Lane 1:

Human small intestine cell lysate at 10 µg

Lane 2:

Human colon cancer cell lysate at 10 µg

Lane 3:

HT-29 cell lysate at 10 µg

Predicted band size: 92 kDa

false

• WB

Unknown

Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID : 18552820).

All lanes:

Western blot - Anti-LI Cadherin antibody [EPR3996] (<a href='/en-us/products/primary-antibodies/li-cadherin-antibody-epr3996-ab109190'>ab109190</a>) at 1/1000 dilution

Lane 1:

LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human small intestine lysate at 20 µg

Lane 3:

Human colon lysate at 20 µg

Lane 4:

Human spleen lysate at 20 µg

Lane 5:

Human kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 92 kDa

Observed band size: 120 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

ab109190 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

ab109190 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

ab109190 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

This data was developed using ab109190, the same antibody clone in a different buffer formulation.

ab109190 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (AB247786)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.