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AB240266

Anti-LI Cadherin antibody [SP183] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LI Cadherin antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cadherin-17, Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin, CDH17

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)

Immunohistochemical analysis of Human colon tissue labeling LI Cadherin using ab183318 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183318).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma
tissue sections labeling LI Cadherin with ab183318 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183318 for 30 mins at room temperature. This image was generated using ab183318, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon
tissue sections labeling LI Cadherin with ab183318 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument
The section was incubated with ab183318 for 30 mins at room temperature. This image was generated using ab183318, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)

Immunohistochemical analysis of Human stomach adenocarcinoma tissue labeling LI Cadherin using ab183318 at a 1/100 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183318).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [SP183] - BSA and Azide free (AB240266)

Immunohistochemical analysis of Human colon adenocarcinoma tissue labeling LI Cadherin using ab183318 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183318).

  • Unconjugated

    Anti-LI Cadherin antibody [SP183] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LI Cadherin antibody [SP183] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LI Cadherin antibody [SP183] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LI Cadherin antibody [SP183] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-LI Cadherin antibody [SP183] - C-terminal

  • 578 PE

    PE Anti-LI Cadherin antibody [SP183] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-LI Cadherin antibody [SP183] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP183

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform Antigen Retrieval by boil tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab240266 is the carrier-free version of ab183318.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LI Cadherin also known as Cadherin-17 is a protein that plays a significant role in cell adhesion specifically in epithelial cells. This protein belongs to the cadherin family and has a molecular mass of approximately 88 kDa. LI Cadherin is mainly expressed in the gastrointestinal tract including areas like the stomach and small intestine. It is found on the surfaces of cells where it facilitates cell-cell interaction by binding with other cadherin proteins on adjacent cells.
Biological function summary

This cadherin protein is important for maintaining the integrity of epithelial layers in the gastrointestinal tract. It contributes to the formation of adherens junctions which are necessary for the maintenance of tissue architecture and cellular communication. As part of a larger protein complex in adherens junctions it interacts with cytoskeletal elements to help stabilize cell structures. This mechanical support ensures that cells remain tightly adhered under various physiological conditions.

Pathways

LI Cadherin is involved in pathways that govern cell adhesion and signal transduction. It participates in the Wnt signaling pathway which is vital for cell proliferation and differentiation. Moreover it interacts with E-cadherin and β-catenin within the Wnt pathway aiding in the regulation of gene expression and cellular morphology. These interactions are important for maintaining normal cell behavior and preventing unwanted cell proliferation.

LI Cadherin's expression has been linked to gastrointestinal cancers particularly gastric and colorectal cancers. Aberrations in cadherin-mediated cell adhesion can lead to tumorigenesis as disrupted cell adhesion can cause increased cell motility and invasion. LI Cadherin also engages with proteins such as α-catenin in tumor cells affecting cellular responses to cancerous transformations. By understanding these relationships researchers aim to develop targeted therapies to address malignancies associated with disrupted cadherin function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
See full target information Cadherin-17
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 3:100595 PubMed37741277

2023

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Phyllis Zixuan Chen,Dario Bressan,Monika Tripathi,Ahmad Miremadi,Massimiliano di Pietro,Lisa M Coussens,Gregory J Hannon,Rebecca C Fitzgerald,Lizhe Zhuang,Young Hwan Chang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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