AB96302

Anti-LIAS antibody

Name: Anti-LIAS antibody (ab96302) | Abcam
Brand: Abcam Limited
SKU: AB96302

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(4 Publications)

Rabbit Polyclonal LIAS antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIAS aa 50-300.

View Alternative Names

LAS, HUSSY-01, LIAS, Lipoate synthase, Lipoic acid synthase, LS, Lip-syn

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Western blot - Anti-LIAS antibody (AB96302)

All lanes:

Western blot - Anti-LIAS antibody (ab96302) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human LIAS aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

O43766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target LIAS also known as lipoic acid synthase plays an important role in the synthesis of lipoic acid. LIAS is an enzyme with a molecular mass of approximately 40 kDa and it is present in many tissues showing high expression in liver and kidney. It functions as a homodimer in the mitochondria indicating its role as an important player in mitochondrial processes. The enzyme catalyzes the final step in the synthesis of lipoic acid by attaching sulfur atoms to octanoyl groups attached to protein domains.

Biological function summary

LIAS contributes to the activation of metabolic complexes by providing protein-bound lipoic acid. It integrates into multiprotein complexes facilitating their function. This lipoic acid acts as a cofactor for key mitochondrial enzyme complexes such as pyruvate dehydrogenase and α-ketoglutarate dehydrogenase which are important for energy production. These complexes are essential for the conversion of nutrients into usable energy highlighting the biochemical importance of LIAS in cellular respiration and metabolic regulation.

Pathways

Lipoic acid synthase is fundamental for mitochondrial energy metabolism and redox regulation pathways. It participates in the tricarboxylic acid cycle where it influences the activity of related enzymes. Through this activity LIAS interacts indirectly with proteins such as pyruvate dehydrogenase and dihydrolipoamide dehydrogenase both of which play a significant role in maintaining cellular energy balance. The efficient functioning of these pathways is important for cellular survival and homeostasis.

Mutations in LIAS have been linked to rare metabolic disorders like multiple mitochondrial dysfunctions syndrome. These mutations can lead to severe energy production deficits due to impaired enzyme complex assembly. In these conditions LIAS's connection to lipoic acid-dependent proteins like pyruvate dehydrogenase impacts disease progression further demonstrating its role in mitochondrial health. On a broader scale alterations in LIAS function might also relate to disorders involving energy metabolism such as neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

See full target information LIAS

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:4059-4072 PubMed39309486

2024

CRISPR-Cas9 gene editing strengthens cuproptosis/chemodynamic/ferroptosis synergistic cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wu,Zijun Bai,Hui Wang,Hanqing Wang,Dahai Hou,Yunzhu Xu,Guanqun Wo,Haibo Cheng,Dongdong Sun,Weiwei Tao

Journal of biochemical and molecular toxicology 38:e23848 PubMed39264832

2024

GAD1 ameliorates glioma progression through regulating cuproptosis via RAS/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Gao,Jing Yang

PLoS genetics 16:e1009176 PubMed33137164

2020

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Emily M Schleicher,Ashna Dhoonmoon,Lindsey M Jackson,Kristen E Clements,Coryn L Stump,Claudia M Nicolae,George-Lucian Moldovan

Haematologica 104:1756-1767 PubMed30765471

2019

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Ki Soon Kim,Gregory Holmes-Hampton,Anamika Singh,Tracey A Rouault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LIAS antibody

Western blot - Anti-LIAS antibody (AB96302)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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