Rabbit Polyclonal LIF antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human LIF.
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- Clinical science (London, England : 1979) 137:367-3832023Interferon regulatory factor 1 (IRF1) inhibits lung endothelial regeneration following inflammation-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaorui Chen,Di Qi,Shulei Fan,Yirui He,Hekun Jing,Daoxin WangPubMed 36857175
- Cancers 14:2022Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Miyazaki,Nobuhito Mori,Yuka Akagi,Tatsuya Oda,Yasuyuki S KidaPubMed 35326527
- Cancer cell international 21:6272021Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKairu Xie,Hairong Xiong,Wen Xiao,Zhiyong Xiong,Wenjun Hu,Jiaxin Ye,Ning Xu,Jian Shi,Changfei Yuan,Zhixian Chen,Daojia Miao,Xiaoping Zhang,Hongmei YangPubMed 34838029
- The journal of obstetrics and gynaecology research 47:3223-32312021Effects of bone marrow mesenchymal stem cells on repair and receptivity of damaged endometrium in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuiling Wang,Chun'e Ren,Junyi JiangPubMed 34184363
- Cell death & disease 12:4172021Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Chen,Qianyan Liu,Yunsong Zhang,Shiying Li,Sheng YiPubMed 33888681
- Evidence-based complementary and alternative medicine : eCAM 2020:94672712020Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoyu Li,Yongcheng Wang,Yuehua Jiang,Dufang Ma,Ping Jiang,Guofeng Zhou,Jinlong Yang,Feng Dong,Hengyi Zhao,Yimei Zhang,Xiao LiPubMed 33628295
- Proceedings of the National Academy of Sciences of the United States of America 117:29035-290452020The NF-κB/leukemia inhibitory factor/STAT3 signaling pathway in antibody-mediated suppression of Sindbis virus replication in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJane X Yeh,Kimberly L W Schultz,Valerie Calvert,Emanuel F Petricoin,Diane E GriffinPubMed 33144502
- Journal of cellular and molecular medicine 24:13196-132072020Remifentanil up-regulates HIF1α expression to ameliorate hepatic ischaemia/reperfusion injury via the ZEB1/LIF axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongsheng Zhou,Shuang Li,Xiaopeng Mei,Tao Jiang,Qiang WangPubMed 32996684
- Life sciences 260:1184392020The therapeutic effects and underlying mechanisms of the intrauterine perfusion of granulocyte colony-stimulating factor on a thin-endometrium rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiran Xie et. al.PubMed 32950574
- FEBS open bio :2020Adipose-derived mesenchymal stem cells differentiate into pancreatic cancer-associated fibroblasts in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Miyazaki et. al.PubMed 32931156
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LIF. The exact immunogen used to generate this antibody is proprietary information. Database link P15018
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Alternative names
HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI
Recommended products
Rabbit Polyclonal LIF antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human LIF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LIF. The exact immunogen used to generate this antibody is proprietary information. Database link P15018
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab113262 is purified by affinity chromatography via a peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.
- Biological function summary
LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.
- Pathways
The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.
- Associated diseases and disorders
LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Western blot - Anti-LIF antibody (ab113262)
Diluting buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Lane 2: Mouse colon tissue lysate at 15 µg
Lane 3: EL4 (mouse lymphoma cell line) cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LIF antibody (ab113262)
Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml.
Western blot - Anti-LIF antibody (ab113262)
Lane 1: Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL
Lane 2: Western blot - Anti-LIF antibody (ab113262) at 2 µg/mL
All lanes: 3T3 cell lysate at 15 µg
Predicted band size: 22 kDa
Western blot - Anti-LIF antibody (ab113262)
Diluting buffer: 5% NFDM/TBST.Lane 1: Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL
Lane 2: Western blot - Anti-LIF antibody (ab113262) at 2 µg/mL
All lanes: Rat lung tissue lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-LIF antibody (ab113262)
LIF western blot using anti-LIF antibody ab113262. Publication image and figure legend from Fioravante, M., Bombassaro, B., et al., 2017, J Neuroinflammation, PubMed 28865476.
ab113262 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab113262 please see the product overview.
Impact of inhibiting hypothalamic LIF on inflammatory markers. a Schematic representation of the protocol employed to immunoneutralize hypothalamic LIF. b The expression of LIF protein was evaluated by immunoblot in the hypothalamus of obesity-resistant (OR) mice treated with non-immune IgG or anti-LIF antibody for 15 days. Real-time PCR was employed to determine the relative expression of transcripts encoding for TNF-α (c), IL-1β (d), CX3CL1 (fractalkine) (e), cxcl1 (f) IL-6 (g), and IL-10 (h) ccl20 (i) in the hypothalamus of obesity-prone (OP) mice treated with non-immune IgG or OR mice treated either with non-immune IgG or anti-LIF antibody. In b–g, n = 5; *p < 0.05 vs. OP IgG and §p < 0.05 vs. OR IgG
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