Anti-LIF antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LIF antibody (AB113262)

Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml.

• WB

Supplier Data

Western blot - Anti-LIF antibody (AB113262)

Diluting buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL

Lane 2:

Western blot - Anti-LIF antibody (ab113262) at 2 µg/mL

All lanes:

Rat lung tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-LIF antibody (AB113262)

Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Lane 2:

Mouse colon tissue lysate at 15 µg

Lane 3:

EL4 (mouse lymphoma cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 26 kDa

false

• WB

Unknown

Western blot - Anti-LIF antibody (AB113262)

Lane 1:

Western blot - Anti-LIF antibody (ab113262) at 1 µg/mL

Lane 2:

Western blot - Anti-LIF antibody (ab113262) at 2 µg/mL

All lanes:

3T3 cell lysate at 15 µg

Predicted band size: 22 kDa

false

• WB

CiteAb

Western blot - Anti-LIF antibody (AB113262)

LIF western blot using anti-LIF antibody ab113262. Publication image and figure legend from Fioravante, M., Bombassaro, B., et al., 2017, J Neuroinflammation, PubMed 28865476.

ab113262 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab113262 please see the product overview.

Impact of inhibiting hypothalamic LIF on inflammatory markers. a Schematic representation of the protocol employed to immunoneutralize hypothalamic LIF. b The expression of LIF protein was evaluated by immunoblot in the hypothalamus of obesity-resistant (OR) mice treated with non-immune IgG or anti-LIF antibody for 15 days. Real-time PCR was employed to determine the relative expression of transcripts encoding for TNF-α (c), IL-1β (d), CX3CL1 (fractalkine) (e), cxcl1 (f) IL-6 (g), and IL-10 (h) ccl20 (i) in the hypothalamus of obesity-prone (OP) mice treated with non-immune IgG or OR mice treated either with non-immune IgG or anti-LIF antibody. In b–g, n = 5; *p < 0.05 vs. OP IgG and §p < 0.05 vs. OR IgG

false