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Rabbit Recombinant Monoclonal LIF antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (AB320822), expandable thumbnail
  • Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (AB320822), expandable thumbnail
  • Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (AB320822), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Alternative names

HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI

Recommended products

Rabbit Recombinant Monoclonal LIF antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28693-84
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab320822 is the carrier-free version of Anti-LIF antibody [EPR28693-84] ab320821.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.

Biological function summary

LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.

Pathways

The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.

Associated diseases and disorders

LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822), expandable thumbnail

    Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822)

    This data was developed using Anti-LIF antibody [EPR28693-84] ab320821, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The up-regulation of LIF is induced by TNF alpha treatment (PMID: 8895217).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    Exposure time: Lane 1-2: 7 seconds, lane 3-4: 70 seconds

    All lanes: Western blot - Anti-LIF antibody [EPR28693-84] (Anti-LIF antibody [EPR28693-84] ab320821) at 1/1000 dilution

    Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated with 25ng/ml TNF alpha for 24 hours whole cell lysate at 20 µg

    Lane 3: Untreated PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 4: PC-3 treated with 50ng/ml TNF alpha and 300ng/ml BFA for 16 hours, whole cell lysate at 40 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 3 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 32 kDa, 124 kDa

  • Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822), expandable thumbnail

    Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822)

    This data was developed using Anti-LIF antibody [EPR28693-84] ab320821, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-LIF antibody [EPR28693-84] (Anti-LIF antibody [EPR28693-84] ab320821) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

    Lane 2: NIH/3T3 treated with 300ng/ml BFA for 24 hours whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 32 kDa, 124 kDa

    Exposure time: 15s

  • Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822), expandable thumbnail

    Western blot - Anti-LIF antibody [EPR28693-84] - BSA and Azide free (ab320822)

    This data was developed using Anti-LIF antibody [EPR28693-84] ab320821, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-2: 48 seconds; lane3-4: 180 seconds

    All lanes: Western blot - Anti-LIF antibody [EPR28693-84] (Anti-LIF antibody [EPR28693-84] ab320821) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 40 µg

    Lane 2: Human skeletal muscle tissue lysate at 40 µg

    Lane 3: Mouse spleen tissue lysate at 40 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 32 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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