Rat Monoclonal LIF antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Lif.
Images
![Western blot - Anti-LIF antibody [IMG39N7D10] (AB138002), expandable thumbnail](https://content.abcam.com/products/images/lif-antibody-img39n7d10-ab138002--western-blot-img78309.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LIF antibody [IMG39N7D10] (AB138002)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (AB138002), expandable thumbnail](https://content.abcam.com/products/images/lif-antibody-img39n7d10-ab138002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70669.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (AB138002)")
Publications
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- Current stem cell research & therapy 18:1001-10122022HOXA10 Expressing UCMSCs Transplantation Improved Endometrial Receptivity on Endometrial Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeixian Wu,Yuanyuan Li,Yiwei Wang,Yifan Li,Jinghui Li,Jing Xie,Shuang Zhao,Lihua SunPubMed 36121094
- Evidence-based complementary and alternative medicine : eCAM 2022:17900412022Acupuncture Improves Endometrial Angiogenesis by Activating PI3K/AKT Pathway in a Rat Model with PCOS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiwei Xing,Yang Chen,Zhe He,Ming He,Yuhuan Sun,Jinlong Xu,Jian Wang,Haina Zhuang,Zeqin Ren,Ying Chen,Jun Yang,Shuluo Cheng,Rong ZhaoPubMed 36062171
- Cell death & disease 13:5172022CircSCAF8 promotes growth and metastasis of prostate cancer through the circSCAF8-miR-140-3p/miR-335-LIF pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao He,Wen Tao,Lei-Lei Zhang,Bang-Yu Wang,Ke Li,Hui-Min Lu,Guo-Jun Tang,Ya-Di He,Liao-Yuan LiPubMed 35654787
- Cell cycle (Georgetown, Tex.) 21:1932-19442022LncRNA NEAT1 affects endometrial receptivity by regulating HOXA10 promoter activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxuan Geng,Chenchen Cui,Yisha Yin,Yan Zhao,Cuilian ZhangPubMed 35574918
- BMC musculoskeletal disorders 23:872022Osteoclasts secrete leukemia inhibitory factor to promote abnormal bone remodeling of subchondral bone in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zhao,Long Ma,Haohui Guo,Jian Wang,Shuai Zhang,Xiaochun Yang,Lvlin Yang,Qunhua JinPubMed 35078447
- Molecular cancer 21:242022circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChonghui Hu,Renpeng Xia,Xiang Zhang,Tingting Li,Yuancheng Ye,Guolin Li,Rihua He,Zhihua Li,Qing Lin,Shangyou Zheng,Rufu ChenPubMed 35045883
- Communications biology 5:222022SMURF2 phosphorylation at Thr249 modifies glioma stemness and tumorigenicity by regulating TGF-β receptor stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManami Hiraiwa,Kazuya Fukasawa,Takashi Iezaki,Hemragul Sabit,Tetsuhiro Horie,Kazuya Tokumura,Sayuki Iwahashi,Misato Murata,Masaki Kobayashi,Akane Suzuki,Gyujin Park,Katsuyuki Kaneda,Tomoki Todo,Atsushi Hirao,Mitsutoshi Nakada,Eiichi HinoiPubMed 35017630
- Frontiers in nutrition 8:6671302021β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyan Yu,Ying Cao,Wenling Huang,Yanxia Liu,Ying Lu,Jiajing ZhaoPubMed 34179058
- Annals of the New York Academy of Sciences 1484:74-892020Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR-Hippo-YAP pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Bo Bian,Yun Yang,Wen-Quan Liang,Ke-Cheng Zhang,Lin Chen,Zhong-Tao ZhangPubMed 32827446
- Lab on a chip 18:3230-32382018Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorinne Sadlowski,Sarah Balderston,Mandeep Sandhu,Reza Hajian,Chao Liu,Thanhtra P Tran,Michael J Conboy,Jacobo Paredes,Niren Murthy,Irina M Conboy,Kiana AranPubMed 30239548
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Lif. Database link P09056
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 3.00000-5.00000 µg/mL | Notes - |
Species Mouse | Dilution info 3.00000-5.00000 µg/mL | Notes - |
Species Human | Dilution info 3.00000-5.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Alternative names
HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI
Recommended products
Rat Monoclonal LIF antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Lif.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Lif. Database link P09056
- Clone number
- IMG39N7D10
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.
- Biological function summary
LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.
- Pathways
The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.
- Associated diseases and disorders
LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002), expandable thumbnail](https://content.abcam.com/products/images/lif-antibody-img39n7d10-ab138002--western-blot-img78309.jpg "Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)")
Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002)
The LIF protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting.
All lanes: Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002) at 3 µg
Lane 1: Full length recombinant LIF protein
Lane 2: Mouse spleen lysate
Lane 3: Human spleen lysate
SecondaryAll lanes: Goat anti-Rat Ig HRP
Developed using the ECL technique.
Predicted band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002), expandable thumbnail](https://content.abcam.com/products/images/lif-antibody-img39n7d10-ab138002--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70669.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (ab138002)
Immunohistochemical analysis of LIF in Mouse colon tissue probed with ab138002 at 5 μg/ml.
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