Anti-LIF antibody [IMG39N7D10]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIF antibody [IMG39N7D10] (AB138002)

Immunohistochemical analysis of LIF in Mouse colon tissue probed with ab138002 at 5 μg/ml.

• WB

Lab

Western blot - Anti-LIF antibody [IMG39N7D10] (AB138002)

Western blot : Rat Monoclonal [IMG39N7D10] to LIF ab138002 staining at 1/1000 dilution, shown in green; Rabbit anti GAPDH ab181602 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 65-80 kDa. The identity of the band detected at ~30kDa is unknown. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rat 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LIF antibody [IMG39N7D10] (ab138002) at 1/1000 dilution

Lane 1:

THP-1 at 20 µg

Lane 2:

PANC-1 at 20 µg

Lane 3:

PANC-1 Supernatant at 15 µg

Lane 4:

BT-549 at 20 µg

Lane 5:

BT-549 Supernatant at 15 µg

Secondary

All lanes:

Goat anti-Rat 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 65-80 kDa,36 kDa

false