AB202847

Anti-LIFR antibody

Name: Anti-LIFR antibody (ab202847) | Abcam
Brand: Abcam Limited
SKU: AB202847
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal LIFR antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Rat Lifr aa 500-550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD118, Leukemia inhibitory factor receptor, LIF receptor, LIF-R, LIFR

3 Images

Flow Cytometry - Anti-LIFR antibody (AB202847)

Flow Cyt

Unknown

Flow Cytometry - Anti-LIFR antibody (AB202847)

Flow Cytometry of Human U937 cells with ab216508 at 1/100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIFR antibody (AB202847)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIFR antibody (AB202847)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinomal tissue labeling LIFR with ab202847 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIFR antibody (AB202847)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinomal tissue labeling LIFR with ab202847 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Rat Lifr aa 500-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O70535

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The leukemia inhibitory factor receptor also known as LIFR is a protein that plays an important role in cellular signaling. It has a mass of approximately 190-210 kDa and it is widely expressed in various tissues such as the liver muscle and bone. LIFR is a member of the interleukin 6 (IL-6) receptor family. The 'LIFR selector' refers to its ability to bind and select its specific ligand leukemia inhibitory factor (LIF) which initiates signal transduction processes. LIFR expression tends to be higher in developing tissues indicating its involvement during embryogenesis.

Biological function summary

The leukemia inhibitory factor receptor is involved in various cellular processes including differentiation proliferation and survival. When LIF binds to LIFR it forms a complex with gp130 another receptor protein which then activates downstream signaling pathways that regulate these cellular processes. This complex formation is an important component in the maintenance of stem cell pluripotency which is important in early development. The LIFR meaning in this context is essential for translating extracellular signals into cellular responses.

Pathways

The leukemia inhibitory factor receptor plays a critical role in the JAK/STAT signaling pathway. This pathway is significant for transmitting information received from extracellular chemical signals to the cell nucleus resulting in DNA transcription. LIFR closely interacts with other proteins like STAT3 and JAK1 which further propagate the signaling cascade initiated by LIF binding. Another pathway involving LIFR is the MAPK/ERK pathway a major pathway that influences cell growth and differentiation. These pathways highlight the interconnectivity of LIFR in regulating key cellular functions.

LIFR has been linked to cancer progression and osteoporosis. Abnormal LIFR signaling can lead to unchecked cellular proliferation contributing to tumorigenesis in various cancers such as liver and breast cancer. LIFR also interacts with proteins like gp130 and the aforementioned STAT3 in the cancerous context. In osteoporosis altered LIFR expression affects bone metabolism potentially leading to increased bone resorption and weakening. The role of LIFR in these diseases demonstrates its potential as a therapeutic target and its importance in maintaining normal tissue homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

See full target information LIFR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:905212 PubMed36072807

2022

Development and validation of a novel fibroblast scoring model for lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyou Wei,Xuyu Gu,Wentian Zhang

The Journal of biological chemistry 298:101946 PubMed35447114

2022

Leukemia inhibitory factor protects against liver steatosis in nonalcoholic fatty liver disease patients and obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Youwen Yuan,Kangli Li,Fei Teng,Weiwei Wang,Bing Zhou,Xuan Zhou,Jiayang Lin,Xueru Ye,Yajuan Deng,Wenhui Liu,Shenjian Luo,Peizhen Zhang,Deying Liu,Minghua Zheng,Jin Li,Yan Lu,Huijie Zhang

Digestive diseases and sciences 67:2244-2256 PubMed34050852

2021

Lidocaine Suppresses Gastric Cancer Development Through Circ_ANO5/miR-21-5p/LIFR Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Enjian Guan,Hongrong Liu,Ning Xu

Oncogene 40:791-805 PubMed33262462

2020

STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Hsin Lin,Yi-Wen Chang,Min-Xiang Hong,Te-Cheng Hsu,Ko-Chuan Lee,Che Lin,Jia-Lin Lee

BioMed research international 2019:1613820 PubMed30937308

2019

Leukemia Inhibitory Factor Receptor Is Involved in Apoptosis in Rat Astrocytes Exposed to Oxygen-Glucose Deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Huo,Yuying Fan,Hua Wang

View all publications

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