AB203578

Anti-LIGHT/TNFSF14 antibody

Name: Anti-LIGHT/TNFSF14 antibody (ab203578) | Abcam
Brand: Abcam Limited
SKU: AB203578
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal LIGHT/TNFSF14 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF14 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD258, HVEML, LIGHT, UNQ391/PRO726, TNFSF14, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L, Herpesvirus entry mediator ligand

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIGHT/TNFSF14 antibody (AB203578)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIGHT/TNFSF14 antibody (AB203578)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue sections labeling LIGHT/TNFSF14 with ab203578 at a 1/200 dilution. DAB staining.

Supplier Data

Western blot - Anti-LIGHT/TNFSF14 antibody (AB203578)

All lanes:

Western blot - Anti-LIGHT/TNFSF14 antibody (ab203578) at 1/200 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat kidney lysate

Secondary

All lanes:

Goat anti-rabbit-HRP at 1/3000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TNFSF14 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43557

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe please use dilutions of 1/50 - 1/200.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIGHT also known as TNFSF14 is a member of the tumor necrosis factor (TNF) superfamily. It has an approximate mass of 24 kDa. You can find LIGHT expressed in various tissues including lymphoid tissues such as the spleen and lymph nodes. LIGHT serves as a ligand that can bind to receptors like lymphotoxin beta receptor (LTβR) and herpesvirus entry mediator (HVEM). Researchers have studied "mouse LIGHT" and "LIGHT protein" to understand its role in immune regulation. Additionally the term "anti-LIGHT" often refers to tools like antibodies used to investigate or inhibit LIGHT's activity.

Biological function summary

LIGHT plays a role in regulating immune responses and inflammation. It is part of a complex formed when it binds to its receptors influencing the proliferation and survival of immune cells such as T cells. LIGHT's interaction with HVEM can modulate T cell signaling cytokine production and dendritic cell maturation. It contributes to the organization of secondary lymphoid organs impacting immune surveillance and homeostasis. The presence of "APC LIGHT" indicates its involvement in antigen-presenting cell activities essential for adaptive immunity.

Pathways

LIGHT operates within the TNF receptor superfamily signaling pathway. This pathway involves other TNF members like TNF-α and lymphotoxin alfa which can also interact with LTβR. Another relevant pathway is the NF-kB signaling cascade which LIGHT activation can trigger. This cascade regulates gene expression related to immune response and cell survival. In connection to these pathways LIGHT's interaction with proteins like HVEM and LTβR helps maintain immune system balance.

LIGHT has links to autoimmune diseases and cancer. Abnormal LIGHT signaling can contribute to conditions like rheumatoid arthritis where immune cell regulation becomes dysregulated. In cancer LIGHT may play a dual role by promoting tumor immunity or conversely tumor progression depending on the context of its expression and receptor interaction. The LIGHT interaction network includes TNFRSF members and can influence pathological inflammatory responses when disrupted.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM (PubMed : 10754304, PubMed : 9462508). Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (PubMed : 10754304).

See full target information TNFSF14

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Ophthalmology and therapy 10:63-74 PubMed33188486

2020

Expression of LIGHT/TNFSF14 and Its Receptors, HVEM and LTβR, Correlates with the Severity of Fibrosis in Lacrimal Sacs from Patients with Lacrimal Duct Obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Pawel Bielecki,Ewa Gindzienska-Sieskiewicz,Joanna Reszeć,Bartosz Piszczatowski,Marek Rogowski,Otylia Kowal-Bielecka,Krzysztof Kowal,Andrzej Sieskiewicz

Molecular medicine reports 17:898-910 PubMed29115500

2017

Roles and mechanisms of TRPC3 and the PLCγ/PKC/CPI-17 signaling pathway in regulating parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Dongming Zheng,Hong Cui,Sishi Liu,Lijuan Zhang,Caixia Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIGHT/TNFSF14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIGHT/TNFSF14 antibody (AB203578)

Western blot - Anti-LIGHT/TNFSF14 antibody (AB203578)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise