AB233502

Anti-LIGHT/TNFSF14 antibody

Name: Anti-LIGHT/TNFSF14 antibody (ab233502) | Abcam
Brand: Abcam Limited
SKU: AB233502
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal LIGHT/TNFSF14 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse, Recombinant fragment - Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tnfsf14 aa 50 to C-terminus.

View Alternative Names

CD258, Light, Tnfsf14, Tumor necrosis factor ligand superfamily member 14

3 Images

Sandwich ELISA - Anti-LIGHT/TNFSF14 antibody (AB233502)

sELISA

Supplier Data

Sandwich ELISA - Anti-LIGHT/TNFSF14 antibody (AB233502)

ab233502, in conjunction a Biotinylated Anti-Murine LIGHT/TNFSF14 as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Murine LIGHT/TNFSF14.

Supplier Data

Western blot - Anti-LIGHT/TNFSF14 antibody (AB233502)

Non-reducing conditions..

All lanes:

Western blot - Anti-LIGHT/TNFSF14 antibody (ab233502) at 0.1 µg/mL

Lane 1:

Recombinant mouse LIGHT/TNFSF14 protein, 250 ng

Lane 2:

Recombinant mouse LIGHT/TNFSF14 protein, 125 ng

Lane 3:

Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng

Lane 4:

Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng

Lane 5:

Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng

Lane 6:

Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng

Lane 7:

Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng

Lane 8:

Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng

Lane 9:

Recombinant mouse LIGHT/TNFSF14 protein, 0.98 ng

Lane 10:

Recombinant mouse LIGHT/TNFSF14 protein, 0.49 ng

Lane 11:

Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng

Predicted band size: 26 kDa

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Supplier Data

Western blot - Anti-LIGHT/TNFSF14 antibody (AB233502)

Reducing conditions.

All lanes:

Western blot - Anti-LIGHT/TNFSF14 antibody (ab233502) at 0.2 µg/mL

Lane 1:

Recombinant mouse LIGHT/TNFSF14 protein, 250 ng

Lane 2:

Recombinant mouse LIGHT/TNFSF14 protein, 125 ng

Lane 3:

Recombinant mouse LIGHT/TNFSF14 protein, 62.5 ng

Lane 4:

Recombinant mouse LIGHT/TNFSF14 protein, 31.25 ng

Lane 5:

Recombinant mouse LIGHT/TNFSF14 protein, 15.625 ng

Lane 6:

Recombinant mouse LIGHT/TNFSF14 protein, 7.8 ng

Lane 7:

Recombinant mouse LIGHT/TNFSF14 protein, 3.9 ng

Lane 8:

Recombinant mouse LIGHT/TNFSF14 protein, 1.95 ng

Lane 9:

Recombinant mouse LIGHT/TNFSF14 protein, 0.975 ng

Lane 10:

Recombinant mouse LIGHT/TNFSF14 protein, 0.49 ng

Lane 11:

Recombinant mouse LIGHT/TNFSF14 protein, 0.24 ng

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Tnfsf14 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Purification technique

Affinity purification Immunogen

Purification notes

ab233502 was purified by affinity chromatography employing an immobilized Murine LIGHT/TNFSF14 matrix.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIGHT also known as TNFSF14 is a member of the tumor necrosis factor (TNF) superfamily. It has an approximate mass of 24 kDa. You can find LIGHT expressed in various tissues including lymphoid tissues such as the spleen and lymph nodes. LIGHT serves as a ligand that can bind to receptors like lymphotoxin beta receptor (LTβR) and herpesvirus entry mediator (HVEM). Researchers have studied "mouse LIGHT" and "LIGHT protein" to understand its role in immune regulation. Additionally the term "anti-LIGHT" often refers to tools like antibodies used to investigate or inhibit LIGHT's activity.

Biological function summary

LIGHT plays a role in regulating immune responses and inflammation. It is part of a complex formed when it binds to its receptors influencing the proliferation and survival of immune cells such as T cells. LIGHT's interaction with HVEM can modulate T cell signaling cytokine production and dendritic cell maturation. It contributes to the organization of secondary lymphoid organs impacting immune surveillance and homeostasis. The presence of "APC LIGHT" indicates its involvement in antigen-presenting cell activities essential for adaptive immunity.

Pathways

LIGHT operates within the TNF receptor superfamily signaling pathway. This pathway involves other TNF members like TNF-α and lymphotoxin alfa which can also interact with LTβR. Another relevant pathway is the NF-kB signaling cascade which LIGHT activation can trigger. This cascade regulates gene expression related to immune response and cell survival. In connection to these pathways LIGHT's interaction with proteins like HVEM and LTβR helps maintain immune system balance.

LIGHT has links to autoimmune diseases and cancer. Abnormal LIGHT signaling can contribute to conditions like rheumatoid arthritis where immune cell regulation becomes dysregulated. In cancer LIGHT may play a dual role by promoting tumor immunity or conversely tumor progression depending on the context of its expression and receptor interaction. The LIGHT interaction network includes TNFRSF members and can influence pathological inflammatory responses when disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB and stimulates the proliferation of T-cells. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (By similarity).

See full target information Tnfsf14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:1417 PubMed40898094

2025

KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Li,Qiang Feng,Ya Jiang,Lilin Yang,Hong Fang,Wenmang Xu,Yuanyuan Wang,Xinyan Pan,Julun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIGHT/TNFSF14 antibody

Sandwich ELISA - Anti-LIGHT/TNFSF14 antibody (AB233502)

Western blot - Anti-LIGHT/TNFSF14 antibody (AB233502)

Western blot - Anti-LIGHT/TNFSF14 antibody (AB233502)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise