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AB305235

Anti-LIGHT/TNFSF14 antibody [EPR23428-536]

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Rabbit Recombinant Monoclonal LIGHT/TNFSF14 antibody. Suitable for Flow Cyt and reacts with Human, Mouse samples.

View Alternative Names

CD258, HVEML, LIGHT, UNQ391/PRO726, TNFSF14, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L, Herpesvirus entry mediator ligand

3 Images
Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) treated with 50ng/mL PMA and 500ng/mL Ionomycin for 24 hours (Right) / Untreated control (Left) cells labelling LIGHT/TNFSF14 with ab305235 at 1/500 dilution (0.1ug)/ Left and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab305235. Then stained with anti-CD56 conjugated to BV421.Gated on viable cells.

Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)

Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) treated with 50ng/mL PMA and 500ng/mL Ionomycin for 24 hours cells labelling LIGHT/TNFSF14 with ab305235 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab305235. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.Gated on viable cells.

Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LIGHT/TNFSF14 antibody [EPR23428-536] (AB305235)

Flow cytometric analysis of Mouse blood cells cells labelling LIGHT/TNFSF14 with ab305235 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab305235. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.Gated on viable cells.

  • Carrier free

    Anti-LIGHT/TNFSF14 antibody [EPR23428-536] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LIGHT/TNFSF14 antibody [EPR23428-536]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23428-536

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

AB47677

Anti-TNFRSF14/HVEM antibody

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIGHT also known as TNFSF14 is a member of the tumor necrosis factor (TNF) superfamily. It has an approximate mass of 24 kDa. You can find LIGHT expressed in various tissues including lymphoid tissues such as the spleen and lymph nodes. LIGHT serves as a ligand that can bind to receptors like lymphotoxin beta receptor (LTβR) and herpesvirus entry mediator (HVEM). Researchers have studied "mouse LIGHT" and "LIGHT protein" to understand its role in immune regulation. Additionally the term "anti-LIGHT" often refers to tools like antibodies used to investigate or inhibit LIGHT's activity.
Biological function summary

LIGHT plays a role in regulating immune responses and inflammation. It is part of a complex formed when it binds to its receptors influencing the proliferation and survival of immune cells such as T cells. LIGHT's interaction with HVEM can modulate T cell signaling cytokine production and dendritic cell maturation. It contributes to the organization of secondary lymphoid organs impacting immune surveillance and homeostasis. The presence of "APC LIGHT" indicates its involvement in antigen-presenting cell activities essential for adaptive immunity.

Pathways

LIGHT operates within the TNF receptor superfamily signaling pathway. This pathway involves other TNF members like TNF-α and lymphotoxin alfa which can also interact with LTβR. Another relevant pathway is the NF-kB signaling cascade which LIGHT activation can trigger. This cascade regulates gene expression related to immune response and cell survival. In connection to these pathways LIGHT's interaction with proteins like HVEM and LTβR helps maintain immune system balance.

LIGHT has links to autoimmune diseases and cancer. Abnormal LIGHT signaling can contribute to conditions like rheumatoid arthritis where immune cell regulation becomes dysregulated. In cancer LIGHT may play a dual role by promoting tumor immunity or conversely tumor progression depending on the context of its expression and receptor interaction. The LIGHT interaction network includes TNFRSF members and can influence pathological inflammatory responses when disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM (PubMed : 10754304, PubMed : 9462508). Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (PubMed : 10754304).
See full target information TNFSF14
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com