AB235108

Anti-LILRA3 antibody

Name: Anti-LILRA3 antibody (ab235108) | Abcam
Brand: Abcam Limited
SKU: AB235108
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal LILRA3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LILRA3 aa 200-350.

View Alternative Names

CD85e, ILT6, LIR4, LILRA3, Leukocyte immunoglobulin-like receptor subfamily A member 3, CD85 antigen-like family member E, Immunoglobulin-like transcript 6, Leukocyte immunoglobulin-like receptor 4, Monocyte inhibitory receptor HM43/HM31, ILT-6, LIR-4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRA3 antibody (AB235108)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRA3 antibody (AB235108)

Paraffin-embedded human lung tissue stained for LILRA3 using ab235108 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Supplier Data

Western blot - Anti-LILRA3 antibody (AB235108)

All lanes:

Western blot - Anti-LILRA3 antibody (ab235108) at 1/500 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LILRA3 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q8N6C8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LILRA3 also known as Leukocyte Immunoglobulin-Like Receptor Subfamily A Member 3 is a soluble receptor lacking a transmembrane domain. This protein has a mass of approximately 45 kDa. It is mainly expressed in immune cells such as monocytes macrophages and some lymphocytes. Researchers detect LILRA3 in serum and other body fluids which suggests its potential role outside of cellular membranes.

Biological function summary

LILRA3 modulates immune responses by binding to unknown ligands. It does not associate with any known receptor complex because it does not possess a transmembrane or cytoplasmic domain. LILRA3 may act as a natural antagonist to other LILR receptors by interfering with their ligand interactions. This antagonism helps regulate the functions of the innate and adaptive immune responses by competing with membrane-bound LILR forms for binding with common ligands.

Pathways

LILRA3 influences the pathways involved in immune regulation including the modulation of cytokine production. It has been linked to pathways associated with the inhibition of inflammation intersecting with receptors like LILRA2 and LILRB1 which are also part of the LILR family. These interactions are essential in balancing immune responses possibly by inhibiting excessive activation that could lead to inflammation or autoimmunity.

Researchers have studied LILRA3 in the context of autoimmune conditions and inflammatory disorders. Decreased levels of LILRA3 expression or activity have associations with diseases such as rheumatoid arthritis and multiple sclerosis. The interaction between LILRA3 and other family members like LILRB2 may influence disease processes by affecting immune regulation and the balance of activating and inhibitory signals within the immune system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

See full target information LILRA3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LILRA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRA3 antibody (AB235108)

Western blot - Anti-LILRA3 antibody (AB235108)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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