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AB200648

Anti-LILRB1 antibody

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(1 Publication)

Rabbit Polyclonal LILRB1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LILRB1 aa 200-300.

View Alternative Names

CD85j, ILT2, LIR1, MIR7, LILRB1, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, Leukocyte immunoglobulin-like receptor 1, CD85 antigen-like family member J, Immunoglobulin-like transcript 2, Monocyte/macrophage immunoglobulin-like receptor 7, ILT-2, MIR-7

1 Images
Western blot - Anti-LILRB1 antibody (AB200648)
  • WB

Supplier Data

Western blot - Anti-LILRB1 antibody (AB200648)

All lanes:

Western blot - Anti-LILRB1 antibody (ab200648)

Lane 1:

HEK293T whole cell lysate

Lane 2:

sp2/0 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human LILRB1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q8NHL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We observe a band in WB for mouse but the band maybe not the homologous protein detected in human.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We observe a band in WB for mouse but the band maybe not the homologous protein detected in human.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We observe a band in WB for mouse but the band maybe not the homologous protein detected in human.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity of ab200648 is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LILRB1 also known as leukocyte immunoglobulin-like receptor subfamily B member 1 or CD85j is a protein expressed mainly on myeloid cells including monocytes and dendritic cells as well as some subsets of lymphocytes. This transmembrane protein is approximately 110-120 kDa in mass and is recognized for its role in immune regulation. LILRB1 interacts with MHC class I molecules on target cells mediating inhibitory signals that prevent immune overactivation. These interactions allow LILRB1 to help maintain self-tolerance and regulate adaptive immune responses.
Biological function summary

LILRB1 functions as an inhibitory receptor that dampens immune system activation by recruiting SHP-1 and SHP-2 phosphatases. As part of a complex with other immune checkpoint molecules LILRB1 modulates the balance between activating and inhibitory signals in immune cells. Its inhibitory signaling prevents excessive inflammation by reducing cytokine production and limiting T cell proliferation.

Pathways

This protein plays a role in immune signaling pathways specifically through interactions with proteins involved in the regulation of immune responses such as KIRs (Killer-cell Immunoglobulin-like Receptors). It participates in pathways that control the inhibitory signals controlling immune activity like the NK cell cytotoxicity pathway and the Fc gamma R-mediated phagocytosis pathway. Through these mechanisms LILRB1 maintains immune equilibrium and preserves tissue integrity during immune responses.

The dysregulation of LILRB1 activity links to autoimmune conditions and infectious disease outcomes. Aberrant expression or function can impact diseases such as rheumatoid arthritis and HIV influencing the progression and severity of these conditions. In rheumatoid arthritis altered LILRB1 signaling may exacerbate inflammation while in the context of HIV it could affect immune cell exhaustion. Furthermore LILRB1 interactions with proteins like ILT2 highlight its potential involvement in modulating immune responses during these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed : 16455647, PubMed : 28636952). Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 11907092, PubMed : 9285411, PubMed : 9842885). Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (PubMed : 16455647). Upon interaction with peptide-bound HLA-G-B2M complex, triggers secretion of growth-promoting factors by decidual NK cells (PubMed : 19304799, PubMed : 29262349). Reprograms B cells toward an immune suppressive phenotype (PubMed : 24453251).
See full target information LILRB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:5301-5324 PubMed36882085

2023

Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Milenkovic,Helaine Graziele Santos Vieira,Morghan C Lucas,Jorge Ruiz-Orera,Giannino Patone,Scott Kesteven,Jianxin Wu,Michael Feneley,Guadalupe Espadas,Eduard Sabidó,Norbert Hübner,Sebastiaan van Heesch,Mirko Völkers,Eva Maria Novoa
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Product promise

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