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AB229186

Anti-LILRB1 antibody [EPR21007]

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(4 Publications)

Rabbit Recombinant Monoclonal LILRB1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD85j, ILT2, LIR1, MIR7, LILRB1, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, Leukocyte immunoglobulin-like receptor 1, CD85 antigen-like family member J, Immunoglobulin-like transcript 2, Monocyte/macrophage immunoglobulin-like receptor 7, ILT-2, MIR-7

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (AB229186)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (AB229186)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in Kupffer cells of human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (AB229186)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB1 antibody [EPR21007] (AB229186)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LILRB1 with ab229186 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and membranous staining in tumor-infiltrating immunocytes is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-LILRB1 antibody [EPR21007] (AB229186)
  • WB

Supplier Data

Western blot - Anti-LILRB1 antibody [EPR21007] (AB229186)

Blocking/diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-LILRB1 antibody [EPR21007] (ab229186) at 1/1000 dilution

Lane 1:

Human fetal spleen at 10 µg

Lane 2:

Human tonsil at 10 µg

Lane 3:

IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lane 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 71 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-LILRB1 antibody [EPR21007] - BSA and Azide free

  • 660 APC

    APC Anti-LILRB1 antibody [EPR21007]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LILRB1 antibody [EPR21007]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LILRB1 antibody [EPR21007]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-LILRB1 antibody [EPR21007]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LILRB1 antibody [EPR21007]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-LILRB1 antibody [EPR21007]

  • 578 PE

    PE Anti-LILRB1 antibody [EPR21007]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21007

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LILRB1 also known as leukocyte immunoglobulin-like receptor subfamily B member 1 or CD85j is a protein expressed mainly on myeloid cells including monocytes and dendritic cells as well as some subsets of lymphocytes. This transmembrane protein is approximately 110-120 kDa in mass and is recognized for its role in immune regulation. LILRB1 interacts with MHC class I molecules on target cells mediating inhibitory signals that prevent immune overactivation. These interactions allow LILRB1 to help maintain self-tolerance and regulate adaptive immune responses.
Biological function summary

LILRB1 functions as an inhibitory receptor that dampens immune system activation by recruiting SHP-1 and SHP-2 phosphatases. As part of a complex with other immune checkpoint molecules LILRB1 modulates the balance between activating and inhibitory signals in immune cells. Its inhibitory signaling prevents excessive inflammation by reducing cytokine production and limiting T cell proliferation.

Pathways

This protein plays a role in immune signaling pathways specifically through interactions with proteins involved in the regulation of immune responses such as KIRs (Killer-cell Immunoglobulin-like Receptors). It participates in pathways that control the inhibitory signals controlling immune activity like the NK cell cytotoxicity pathway and the Fc gamma R-mediated phagocytosis pathway. Through these mechanisms LILRB1 maintains immune equilibrium and preserves tissue integrity during immune responses.

The dysregulation of LILRB1 activity links to autoimmune conditions and infectious disease outcomes. Aberrant expression or function can impact diseases such as rheumatoid arthritis and HIV influencing the progression and severity of these conditions. In rheumatoid arthritis altered LILRB1 signaling may exacerbate inflammation while in the context of HIV it could affect immune cell exhaustion. Furthermore LILRB1 interactions with proteins like ILT2 highlight its potential involvement in modulating immune responses during these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed : 16455647, PubMed : 28636952). Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 11907092, PubMed : 9285411, PubMed : 9842885). Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (PubMed : 16455647). Upon interaction with peptide-bound HLA-G-B2M complex, triggers secretion of growth-promoting factors by decidual NK cells (PubMed : 19304799, PubMed : 29262349). Reprograms B cells toward an immune suppressive phenotype (PubMed : 24453251).
See full target information LILRB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International dental journal 75:100872 PubMed40543136

2025

SOX11 and GLIS2: Novel Biomarkers for Understanding the Progression of Oral Leukoplakia.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Zhencun Tang,Chenliang Xiao,Jun Su,Lijia He,Wenyun Zhang

EMBO molecular medicine 16:1755-1790 PubMed39030302

2024

LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Qiyao Chai,Zehui Lei,Yiru Wang,Jiehua He,Pupu Ge,Zhe Lu,Lihua Qiang,Dongdong Zhao,Shanshan Yu,Changgen Qiu,Yanzhao Zhong,Bing-Xi Li,Lingqiang Zhang,Yu Pang,George Fu Gao,Cui Hua Liu

World journal of surgical oncology 20:92 PubMed35321724

2022

Analysis of the expression and prognosis for leukocyte immunoglobulin-like receptor subfamily B in human liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Fan,Lili Wang,Miao Chen,Jiakang Zhang,Jiayan Li,Fangnan Song,Aidong Gu,Dandan Yin,Yongxiang Yi

Nature : PubMed32650338

2020

Structural basis for RIFIN-mediated activation of LILRB1 in malaria.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas E Harrison,Alexander M Mørch,James H Felce,Akihito Sakoguchi,Adam J Reid,Hisashi Arase,Michael L Dustin,Matthew K Higgins
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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