AB185325

Anti-LILRB1 antibody [GHI/75]

Name: Anti-LILRB1 antibody [GHI/75] (ab185325) | Abcam
Brand: Abcam Limited
SKU: AB185325
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal LILRB1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing LILRB1 protein.

View Alternative Names

CD85j, ILT2, LIR1, MIR7, LILRB1, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, Leukocyte immunoglobulin-like receptor 1, CD85 antigen-like family member J, Immunoglobulin-like transcript 2, Monocyte/macrophage immunoglobulin-like receptor 7, ILT-2, MIR-7

3 Images

Flow Cytometry - Anti-LILRB1 antibody [GHI/75] (AB185325)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-LILRB1 antibody [GHI/75] (AB185325)

Flow cytometry analysis of human peripheral blood labeling CD85j/LILRB1 using ab185325 at 1 µg/mL. CD19 is labeled using an Anti_human CD19-PE conjugate at 20 µL/100ul blood. Secondary antibody is a Goat Anti-Mouse APB conjugate. Surface staining.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-LILRB1 antibody [GHI/75] (AB185325)

Flow cytometry analysis of human peripheral blood labeling CD85j/LILRB1 using ab185325 at 1 µg/mL. Secondary antibody is a Goat Anti-Mouse APB conjugate. Surface staining.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-LILRB1 antibody [GHI/75] (AB185325)

Flow cytometry analysis of human peripheral blood labeling CD85j/LILRB1 using ab185325 at 1 µg/mL. CD85j/LILRB1-positive B-cells (Red) are separated from CD85j/LILRB1-negative neutrophil granulocytes (Black, dashed line). Secondary antibody is a Goat Anti-Mouse APB conjugate. Surface staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GHI/75

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing LILRB1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8NHL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LILRB1 also known as leukocyte immunoglobulin-like receptor subfamily B member 1 or CD85j is a protein expressed mainly on myeloid cells including monocytes and dendritic cells as well as some subsets of lymphocytes. This transmembrane protein is approximately 110-120 kDa in mass and is recognized for its role in immune regulation. LILRB1 interacts with MHC class I molecules on target cells mediating inhibitory signals that prevent immune overactivation. These interactions allow LILRB1 to help maintain self-tolerance and regulate adaptive immune responses.

Biological function summary

LILRB1 functions as an inhibitory receptor that dampens immune system activation by recruiting SHP-1 and SHP-2 phosphatases. As part of a complex with other immune checkpoint molecules LILRB1 modulates the balance between activating and inhibitory signals in immune cells. Its inhibitory signaling prevents excessive inflammation by reducing cytokine production and limiting T cell proliferation.

Pathways

This protein plays a role in immune signaling pathways specifically through interactions with proteins involved in the regulation of immune responses such as KIRs (Killer-cell Immunoglobulin-like Receptors). It participates in pathways that control the inhibitory signals controlling immune activity like the NK cell cytotoxicity pathway and the Fc gamma R-mediated phagocytosis pathway. Through these mechanisms LILRB1 maintains immune equilibrium and preserves tissue integrity during immune responses.

The dysregulation of LILRB1 activity links to autoimmune conditions and infectious disease outcomes. Aberrant expression or function can impact diseases such as rheumatoid arthritis and HIV influencing the progression and severity of these conditions. In rheumatoid arthritis altered LILRB1 signaling may exacerbate inflammation while in the context of HIV it could affect immune cell exhaustion. Furthermore LILRB1 interactions with proteins like ILT2 highlight its potential involvement in modulating immune responses during these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed : 16455647, PubMed : 28636952). Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed : 11907092, PubMed : 9285411, PubMed : 9842885). Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (PubMed : 16455647). Upon interaction with peptide-bound HLA-G-B2M complex, triggers secretion of growth-promoting factors by decidual NK cells (PubMed : 19304799, PubMed : 29262349). Reprograms B cells toward an immune suppressive phenotype (PubMed : 24453251).

See full target information LILRB1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38625739

2024

The secreted micropeptide C4orf48 enhances renal fibrosis via an RNA-binding mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yang,Hongjie Zhuang,Jinhua Li,Ana B Nunez-Nescolarde,Ning Luo,Huiting Chen,Andy Li,Xinli Qu,Qing Wang,Jinjin Fan,Xiaoyan Bai,Zhiming Ye,Bing Gu,Yue Meng,Xingyuan Zhang,Di Wu,Youyang Sia,Xiaoyun Jiang,Wei Chen,Alexander N Combes,David J Nikolic-Paterson,Xueqing Yu

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