Anti-LILRB5 antibody [EPR23896-84]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) cells labelling LILRB5 with ab313612 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human LILRB5 expression vector containing a myc tag (Right) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-Myc conjugated to 647.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling LILRB5 with ab313612 at 1/2000 (0.255 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with a human LILRB5 expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : 293T. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 114 seconds

All lanes:

Western blot - Anti-LILRB5 antibody [EPR23896-84] (ab313612) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 60 µg

Lane 2:

No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-LILRB5 antibody [EPR23896-84] (AB313612)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human LILRB1, LILRB2, LILRB3, and LILRB4. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 103 seconds

All lanes:

Western blot - Anti-LILRB5 antibody [EPR23896-84] (ab313612) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag whole cell lysate at 60 µg

Lane 2:

293T cells transfected with a human LILRB3 expression vector containing a His-tag whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a human LILRB1 expression vector containing a His-tag whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human LILRB5 expression vector containing a His-tag whole cell lysate at 60 µg

Lane 5:

293T cells transfected with a human LILRB2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human LILRB4 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 103s