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AB230490

Anti-LIM domain only 3 antibody

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(7 Publications)

Rabbit Polyclonal LIM domain only 3 antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human LMO3 aa 50 to C-terminus.

View Alternative Names

RBTN3, RBTNL2, RHOM3, LMO3, LIM domain only protein 3, LMO-3, Neuronal-specific transcription factor DAT1, Rhombotin-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM domain only 3 antibody (AB230490)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM domain only 3 antibody (AB230490)

Formalin-fixed, paraffin-embedded human testis tissue stained for LIM domain only 3 with ab230490 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-LIM domain only 3 antibody (AB230490)
  • WB

Supplier Data

Western blot - Anti-LIM domain only 3 antibody (AB230490)

All lanes:

Western blot - Anti-LIM domain only 3 antibody (ab230490) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 cell extract with Immunizing peptide

Predicted band size: 17 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LMO3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TAP4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as LIM domain only 3 (LMO3) plays an important role in transcriptional regulation. Alternative names include LMO-3. It has a molecular mass of around 19 kDa. LMO3 contains two LIM domains which are specialized zinc finger-like structures essential for protein-protein interactions. This protein often functions in the nucleus facilitating interactions between different transcriptional machinery components. LMO3 is expressed in various tissues including the central nervous system and some smooth muscle tissues.
Biological function summary

LIM domain only 3 influences cell differentiation and development particularly within neuronal and muscle cells. The presence of LMO3 in these tissues allows it to act as a transcriptional modulator in developmental processes. It works by forming complexes with other proteins including transcription factors to either upregulate or downregulate gene expression. Through these complex formations LMO3 influences vital developmental processes and maintains cellular identity.

Pathways

Researchers recognize LIM domain only 3 as a component of the Wnt signaling pathway and Notch signaling pathway. In the Wnt pathway LMO3 associates with proteins like β-catenin which are important for regulating gene transcription and maintaining cellular homeostasis. In the Notch pathway LMO3 interacts with the Notch receptor influencing decisions on cell fate during development and growth.

The influence of LIM domain only 3 becomes apparent in various cancers and neurodevelopmental disorders. Aberrant expression of LMO3 has connections to certain cancers including neuroblastoma where it interacts with proteins like MYCN leading to enhanced tumorigenesis. Moreover dysregulation in LMO3 expression may contribute to neurodevelopmental disorders affecting normal brain development and function due to its role in cell differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LMO3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 45:475-486 PubMed38366633

2024

LMO3 is a suppressor of the basal-like/squamous subtype and reduces disease aggressiveness of pancreatic cancer through glycerol 3-phosphate metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yuuki Ohara,Amanda J Craig,Huaitian Liu,Shouhui Yang,Paloma Moreno,Tiffany H Dorsey,Helen Cawley,Azadeh Azizian,Jochen Gaedcke,Michael Ghadimi,Nader Hanna,Stefan Ambs,S Perwez Hussain

BMC cancer 22:1364 PubMed36581816

2022

mA RNA methylation regulator-based signature for prognostic prediction and its potential immunological role in uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fang,Peipei Wang,Haoyu Huang,Mingquan Ye,Xiaocen Liu,Qingqing Li

Histology and histopathology 38:797-810 PubMed36426731

2022

Circ_0007385 promotes the proliferation and inhibits the apoptosis of non-small cell lung cancer cells via miR-337-3p-dependent regulation of LMO3.

Applications

Unspecified application

Species

Unspecified reactive species

Mingchao Wei,Rongjiang Yin,Li Qu,Jian Tang

Neurosurgical review 45:2767-2775 PubMed35445910

2022

Expression of decitabine-targeted oncogenes in meningiomas in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Canisius,Andrea Wagner,Eva Christina Bunk,Dorothee Cäcilia Spille,Louise Stögbauer,Oliver Grauer,Katharina Hess,Christian Thomas,Werner Paulus,Walter Stummer,Volker Senner,Benjamin Brokinkel

Open medicine (Warsaw, Poland) 17:453-462 PubMed35350839

2022

LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyi Ling,Xiaoli Long,Ying Wu,Jie Li,Mingliang Feng

Nature communications 12:1453 PubMed33674603

2021

Identification of CD318, TSPAN8 and CD66c as target candidates for CAR T cell based immunotherapy of pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schäfer,Stefan Tomiuk,Laura N Küster,Wa'el Al Rawashdeh,Janina Henze,German Tischler-Höhle,David J Agorku,Janina Brauner,Cathrin Linnartz,Dominik Lock,Andrew Kaiser,Christoph Herbel,Dominik Eckardt,Melina Lamorte,Dorothee Lenhard,Julia Schüler,Philipp Ströbel,Jeannine Missbach-Guentner,Diana Pinkert-Leetsch,Frauke Alves,Andreas Bosio,Olaf Hardt

Cell reports 33:108263 PubMed33053338

2020

Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo J R Fernandes,Nikolaos Patikas,Stefanie Foskolou,Sarah F Field,Jong-Eun Park,Meg L Byrne,Andrew R Bassett,Emmanouil Metzakopian
View all publications
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Product promise

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