Rabbit Polyclonal LIM domain only 3 antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human LMO3 aa 50 to C-terminus.
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Publications
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- Histology and histopathology 38:797-8102022Circ_0007385 promotes the proliferation and inhibits the apoptosis of non-small cell lung cancer cells via miR-337-3p-dependent regulation of LMO3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingchao Wei,Rongjiang Yin,Li Qu,Jian TangPubMed 36426731
- Neurosurgical review 45:2767-27752022Expression of decitabine-targeted oncogenes in meningiomas in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Canisius,Andrea Wagner,Eva Christina Bunk,Dorothee Cäcilia Spille,Louise Stögbauer,Oliver Grauer,Katharina Hess,Christian Thomas,Werner Paulus,Walter Stummer,Volker Senner,Benjamin BrokinkelPubMed 35445910
- Open medicine (Warsaw, Poland) 17:453-4622022LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeyi Ling,Xiaoli Long,Ying Wu,Jie Li,Mingliang FengPubMed 35350839
- Cell reports 33:1082632020Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugo J R Fernandes,Nikolaos Patikas,Stefanie Foskolou,Sarah F Field,Jong-Eun Park,Meg L Byrne,Andrew R Bassett,Emmanouil MetzakopianPubMed 33053338
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LMO3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TAP4
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| African green monkey | Expected | Tested |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish, Orangutan, Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish, Orangutan, Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Alternative names
RBTN3, RBTNL2, RHOM3, LMO3, LIM domain only protein 3, LMO-3, Neuronal-specific transcription factor DAT1, Rhombotin-3
Recommended products
Rabbit Polyclonal LIM domain only 3 antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human LMO3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LMO3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TAP4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as LIM domain only 3 (LMO3) plays an important role in transcriptional regulation. Alternative names include LMO-3. It has a molecular mass of around 19 kDa. LMO3 contains two LIM domains which are specialized zinc finger-like structures essential for protein-protein interactions. This protein often functions in the nucleus facilitating interactions between different transcriptional machinery components. LMO3 is expressed in various tissues including the central nervous system and some smooth muscle tissues.
- Biological function summary
LIM domain only 3 influences cell differentiation and development particularly within neuronal and muscle cells. The presence of LMO3 in these tissues allows it to act as a transcriptional modulator in developmental processes. It works by forming complexes with other proteins including transcription factors to either upregulate or downregulate gene expression. Through these complex formations LMO3 influences vital developmental processes and maintains cellular identity.
- Pathways
Researchers recognize LIM domain only 3 as a component of the Wnt signaling pathway and Notch signaling pathway. In the Wnt pathway LMO3 associates with proteins like β-catenin which are important for regulating gene transcription and maintaining cellular homeostasis. In the Notch pathway LMO3 interacts with the Notch receptor influencing decisions on cell fate during development and growth.
- Associated diseases and disorders
The influence of LIM domain only 3 becomes apparent in various cancers and neurodevelopmental disorders. Aberrant expression of LMO3 has connections to certain cancers including neuroblastoma where it interacts with proteins like MYCN leading to enhanced tumorigenesis. Moreover dysregulation in LMO3 expression may contribute to neurodevelopmental disorders affecting normal brain development and function due to its role in cell differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LIM domain only 3 antibody (ab230490)
All lanes: Western blot - Anti-LIM domain only 3 antibody (ab230490) at 1/500 dilution
Lane 1: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2: COS-7 cell extract with Immunizing peptide
Developed using the ECL technique.
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM domain only 3 antibody (ab230490)
Formalin-fixed, paraffin-embedded human testis tissue stained for LIM domain only 3 with ab230490 at 1/100 dilution in immunohistochemical analysis.
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