Rabbit Polyclonal LIM Kinase 1 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human LIMK1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LIMK1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P53667
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11832213, PubMed:12807904, PubMed:15660133, PubMed:16230460, PubMed:18028908, PubMed:22328514, PubMed:23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed:10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PubMed:11832213, PubMed:15660133, PubMed:16230460, PubMed:23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PubMed:11832213, PubMed:15660133, PubMed:16230460, PubMed:23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PubMed:18028908). Stimulates axonal outgrowth and may be involved in brain development (PubMed:18028908). Isoform 3. Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
Alternative names
LIMK, LIMK1, LIM domain kinase 1, LIMK-1
Recommended products
Rabbit Polyclonal LIM Kinase 1 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human LIMK1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LIMK1 aa 450-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P53667
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LIM Kinase 1 also known as LIMK1 is a serine/threonine protein kinase that plays a critical role in regulating actin cytoskeleton dynamics. Weighing approximately 72 kDa LIMK1 is expressed in various tissues with significant levels observed in the brain and spinal cord. Some studies highlight its elevated presence in neurons. The LIMK1 gene encodes the protein and its activity is often regulated through phosphorylation processes. Researchers often refer to related proteins like LIMK1/proteina in discussions about similar functions.
- Biological function summary
This kinase modulates cell shape migration and differentiation by influencing actin filament networks. LIMK1 associates with regulatory complexes and interacts with several proteins to facilitate cytoskeletal reorganization. It plays an essential part in neural development and synaptic plasticity affecting learning and memory. By phosphorylating key actin-binding proteins like cofilin LIMK1 alters actin dynamics essential for various cellular functions.
- Pathways
LIM Kinase 1 influences the RhoA/ROCK and Rac1/Pak pathways both critical for cytoskeletal rearrangements. Within these pathways LIMK1 interacts closely with proteins such as cofilin and is influenced by upstream activators like Rho GTPases. These pathways link it to a broader network of signaling that controls cell movement and stability important for cellular responses in various environmental contexts.
- Associated diseases and disorders
LIMK1 is linked to neurological conditions like Williams-Beuren syndrome and some forms of intellectual disability. Changes in LIMK1 expression or activity can disrupt normal brain function leading to cognitive deficits. Its connection to cofilin and other cytoskeleton-associated proteins plays a role in the pathological development of these conditions underlining its importance in maintaining normal neuronal architecture and connectivity.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody (ab217690)
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling LIM Kinase 1 using ab217690 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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