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Knockout Tested Mouse Monoclonal LIM Kinase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIMK1.

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Images

Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084), expandable thumbnail
  • Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human LIMK1. Database link P53667

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Expected
African green monkey
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11832213, PubMed:12807904, PubMed:15660133, PubMed:16230460, PubMed:18028908, PubMed:22328514, PubMed:23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed:10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PubMed:11832213, PubMed:15660133, PubMed:16230460, PubMed:23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PubMed:11832213, PubMed:15660133, PubMed:16230460, PubMed:23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PubMed:18028908). Stimulates axonal outgrowth and may be involved in brain development (PubMed:18028908). Isoform 3. Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

Alternative names

LIMK, LIMK1, LIM domain kinase 1, LIMK-1

Recommended products

Knockout Tested Mouse Monoclonal LIM Kinase 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIMK1.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human LIMK1. Database link P53667
Clone number
OTI3G3
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from 3G3 to OTI3G3, both clone numbers name the same clone.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LIM Kinase 1 also known as LIMK1 is a serine/threonine protein kinase that plays a critical role in regulating actin cytoskeleton dynamics. Weighing approximately 72 kDa LIMK1 is expressed in various tissues with significant levels observed in the brain and spinal cord. Some studies highlight its elevated presence in neurons. The LIMK1 gene encodes the protein and its activity is often regulated through phosphorylation processes. Researchers often refer to related proteins like LIMK1/proteina in discussions about similar functions.

Biological function summary

This kinase modulates cell shape migration and differentiation by influencing actin filament networks. LIMK1 associates with regulatory complexes and interacts with several proteins to facilitate cytoskeletal reorganization. It plays an essential part in neural development and synaptic plasticity affecting learning and memory. By phosphorylating key actin-binding proteins like cofilin LIMK1 alters actin dynamics essential for various cellular functions.

Pathways

LIM Kinase 1 influences the RhoA/ROCK and Rac1/Pak pathways both critical for cytoskeletal rearrangements. Within these pathways LIMK1 interacts closely with proteins such as cofilin and is influenced by upstream activators like Rho GTPases. These pathways link it to a broader network of signaling that controls cell movement and stability important for cellular responses in various environmental contexts.

Associated diseases and disorders

LIMK1 is linked to neurological conditions like Williams-Beuren syndrome and some forms of intellectual disability. Changes in LIMK1 expression or activity can disrupt normal brain function leading to cognitive deficits. Its connection to cofilin and other cytoskeleton-associated proteins plays a role in the pathological development of these conditions underlining its importance in maintaining normal neuronal architecture and connectivity.

Product promise

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5 product images

  • Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084), expandable thumbnail

    Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)

    All lanes: Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected the pCMV6-ENTRY control at 5 µg

    Lane 2: HEK-293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1 at 5 µg

    Predicted band size: 73 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)

    Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human endometrium adenocarcinoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lymphoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084), expandable thumbnail

    Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)

    Western blot: Anti-LIMK1 antibody [OTI3G3] (ab119084) staining at 1/2000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab119084 was shown to bind specifically to LIMK1. A band was observed at 73 kDa in wild-type A549 cell lysates with no signal observed at this size in LIMK1 knockout cell line. To generate this image, wild-type and LIMK1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: LIMK1 knockout A549 cell lysate at 20 µg

    Lane 3: Wild-type HAP1 cell lysate at 20 µg

    Lane 4: LIMK1 knockout HAP1 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 73 kDa

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Product protocols

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