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AB229474

Anti-LIM1/LHX1 antibody

5

(3 Reviews)

|

(6 Publications)

Rabbit Polyclonal LIM1/LHX1 antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LHX1.

View Alternative Names

LIM-1, LIM1, LHX1, LIM/homeobox protein Lhx1, LIM homeobox protein 1, Homeobox protein Lim-1, hLim-1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-LIM1/LHX1 antibody (AB229474)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LIM1/LHX1 antibody (AB229474)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling LIM1 protein at cytoplasm and nucleus using ab229474 at a 1/250 dilution (Green).
Tau, an axon marker, stained by Tau antibody at a 1/500 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LHX1. The exact immunogen used to generate this antibody is proprietary information.

P48742

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIM1 also known as LHX1 is a member of the LIM homeodomain protein family. Its role involves transcriptional regulation of genes necessary for embryonic development. LIM1 contains a LIM domain—a double zinc finger structure—that allows for protein-protein interactions and a homeodomain necessary for DNA binding. With an approximate mass of 44 kilodaltons LIM1 is expressed in various tissues including the developing brain kidneys and reproductive organs. This multifaceted expression points to its involvement in multiple developmental processes.
Biological function summary

LIM1 serves as a regulator of gene expression critical for proper tissue and organ formation. During early embryogenesis it coordinates the development of structures in the central nervous system and urogenital systems. LIM1 often functions within a transcriptional complex involving other proteins facilitating the activation or repression of target gene transcription. This action is important for the precise spatial and temporal patterning required for organ and tissue differentiation.

Pathways

LIM1 integrates into several signaling pathways that coordinate developmental cues. Two significant pathways include the Wnt and BMP pathways. Within these LIM1 interacts with other key proteins like β-catenin and Smad proteins ensuring proper transmission of developmental signals. These pathways underpin the processes of pattern formation and axis specification positioning LIM1 as a central regulatory node within these systems.

LIM1 has been associated with congenital disorders such as renal malformations and various neurological deficits. Abnormal expression or function of LIM1 can disrupt the developmental processes controlled by its pathways. For instance its interaction with BMP signaling is related to certain nephrogenic disorders. Disorders of the central nervous system have also linked malfunctions with other LIM homeodomain proteins highlighting the broader impact of this protein family on development and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
See full target information LHX1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:34813 PubMed41053290

2025

An integrated transcriptomic approach for identifying enhancers in limb motor pools.

Applications

Unspecified application

Species

Unspecified reactive species

In Seo Yeo,Dojin Jeong,Yunjeong Lee,Jae-Woo Ahn,Se-Eun Bae,Mi-Ryoung Song

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413508 PubMed40307990

2025

NKX2-5/LHX1 and UHRF1 Establishing a Positive Feedback Regulatory Circuitry Drives Esophageal Squamous Cell Carcinoma through Epigenetic Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xukun Li,Dandan Fan,Yong Li,Jian Yuan,Wanyuan Sun,Qinghao Zhu,Ling Qi,Xueling Wu,Jiahui Cai,Tongyang Gong,Ning Zhao,Jianzhong Su,Zhihua Liu,Hongyan Chen

Nature communications 16:2531 PubMed40087269

2025

Expression of ENL YEATS domain tumor mutations in nephrogenic or stromal lineage impairs kidney development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyu Xue,Hongwen Xuan,Kin Lau,Yangzhou Su,Marc Wegener,Kuai Li,Lisa Turner,Marie Adams,Xiaobing Shi,Hong Wen

Heliyon 10:e32457 PubMed39027525

2024

LIM homeobox 1 (LHX1) induces endoplasmic reticulum stress and promotes preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Liyin Qiu,Zhaozhen Liu,Shouzhen Chen,Yiting Wu,Jianying Yan

Science advances 10:eadk2149 PubMed38781326

2024

A global gene regulatory program and its region-specific regulator partition neurons into commissural and ipsilateral projection types.

Applications

Unspecified application

Species

Unspecified reactive species

Aki Masuda,Kazuhiko Nishida,Rieko Ajima,Yumiko Saga,Marah Bakhtan,Avihu Klar,Tatsumi Hirata,Yan Zhu

The Journal of biological chemistry 297:101306 PubMed34673031

2021

Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization.

Applications

Unspecified application

Species

Unspecified reactive species

Sean R Kundinger,Eric B Dammer,Luming Yin,Cheyenne Hurst,Sarah Shapley,Lingyan Ping,Sohail Khoshnevis,Homa Ghalei,Duc M Duong,Nicholas T Seyfried
View all publications
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Product promise

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