Rabbit Polyclonal LIM1/LHX1 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LHX1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LHX1. The exact immunogen used to generate this antibody is proprietary information. Database link P48742
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Predicted | Predicted | Predicted |
| Mouse | Tested | Tested | Tested |
| Rat | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Chimpanzee | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Chimpanzee | Dilution info - | Notes - |
Target data
Function
Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
Alternative names
LIM-1, LIM1, LHX1, LIM/homeobox protein Lhx1, LIM homeobox protein 1, Homeobox protein Lim-1, hLim-1
Recommended products
Rabbit Polyclonal LIM1/LHX1 antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LHX1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LHX1. The exact immunogen used to generate this antibody is proprietary information. Database link P48742
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LIM1 also known as LHX1 is a member of the LIM homeodomain protein family. Its role involves transcriptional regulation of genes necessary for embryonic development. LIM1 contains a LIM domain—a double zinc finger structure—that allows for protein-protein interactions and a homeodomain necessary for DNA binding. With an approximate mass of 44 kilodaltons LIM1 is expressed in various tissues including the developing brain kidneys and reproductive organs. This multifaceted expression points to its involvement in multiple developmental processes.
- Biological function summary
LIM1 serves as a regulator of gene expression critical for proper tissue and organ formation. During early embryogenesis it coordinates the development of structures in the central nervous system and urogenital systems. LIM1 often functions within a transcriptional complex involving other proteins facilitating the activation or repression of target gene transcription. This action is important for the precise spatial and temporal patterning required for organ and tissue differentiation.
- Pathways
LIM1 integrates into several signaling pathways that coordinate developmental cues. Two significant pathways include the Wnt and BMP pathways. Within these LIM1 interacts with other key proteins like β-catenin and Smad proteins ensuring proper transmission of developmental signals. These pathways underpin the processes of pattern formation and axis specification positioning LIM1 as a central regulatory node within these systems.
- Associated diseases and disorders
LIM1 has been associated with congenital disorders such as renal malformations and various neurological deficits. Abnormal expression or function of LIM1 can disrupt the developmental processes controlled by its pathways. For instance its interaction with BMP signaling is related to certain nephrogenic disorders. Disorders of the central nervous system have also linked malfunctions with other LIM homeodomain proteins highlighting the broader impact of this protein family on development and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
Paraffin-embedded rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
Frozen sectioned E13.5 rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: SOX2, stained by SOX2 antibody at 1/250. dilution
Blue: Fluoroshield with DAPI.
Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
Frozen sectioned adult mouse retina tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: Protein kinase C alpha staining.
Blue: Fluorshield with DAPI.
Western blot - Anti-LIM1/LHX1 antibody (ab229474)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-LIM1/LHX1 antibody (ab229474) at 1/2000 dilution
Lane 1: Mouse brain extract at 50 µg
Lane 2: Rat brain extract at 50 µg
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
Paraffin-embedded mouse hind brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
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